Markova, EA;
Zanetti, G;
(2019)
Visualizing membrane trafficking through the electron microscope: cryo-tomography of coat complexes.
Acta Crystallographica Section D: Structural Biology
, 75
(5)
pp. 467-474.
10.1107/S2059798319005011.
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Abstract
Coat proteins mediate vesicular transport between intracellular compartments, which is essential for the distribution of molecules within the eukaryotic cell. The global arrangement of coat proteins on the membrane is key to their function, and cryo-electron tomography and subtomogram averaging have been used to study membrane-bound coat proteins, providing crucial structural insight. This review outlines a workflow for the structural elucidation of coat proteins, incorporating recent developments in the collection and processing of cryo-electron tomography data. Recent work on coat protein I, coat protein II and retromer performed on in vitro reconstitutions or in situ is summarized. These studies have answered long-standing questions regarding the mechanisms of membrane binding, polymerization and assembly regulation of coat proteins.
| Type: | Article |
|---|---|
| Title: | Visualizing membrane trafficking through the electron microscope: cryo-tomography of coat complexes |
| Location: | United States |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1107/S2059798319005011 |
| Publisher version: | https://doi.org/10.1107/S2059798319005011 |
| Language: | English |
| Additional information: | This is an open-access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited. |
| Keywords: | COPII, coat proteins, cryo-electron tomography, subtomogram averaging, three-dimensional reconstruction, vesicular transport |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Structural and Molecular Biology |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10075552 |
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