Deathridge, J;
Antolović, V;
Parsons, M;
Chubb, JR;
(2019)
Live imaging of ERK signaling dynamics in differentiating mouse embryonic stem cells.
Development
, 146
(12)
, Article dev172940. 10.1242/dev.172940.
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Abstract
Stimulation of the ERK/MAPK pathway is required for the exit from pluripotency and onset of differentiation in mouse embryonic stem cells (ESCs). The dynamic behavior of ERK activity in individual cells during this transition is unclear. Using a FRET-based biosensor, we monitored ERK signaling dynamics of single mouse ESCs during differentiation. ERK activity was highly heterogeneous, with considerable variability in ERK signaling between single cells within ESC colonies. Different triggers of differentiation induced distinct ERK activity profiles. Surprisingly, the dynamic features of ERK signaling were not strongly coupled to loss of pluripotency marker expression, regardless of the differentiation stimulus, suggesting the normal dynamic range of ERK signaling is not rate-limiting in single cells during differentiation. ERK signaling dynamics were sensitive to the degree of cell crowding and were similar in neighbouring cells. Sister cells from a mitotic division also showed more similar ERK activity, an effect that was apparent whether cells remained adjacent or moved apart after division. These data suggest a combination of cell lineage and niche contributes to the absolute level of ERK signaling in mouse ESCs.
Type: | Article |
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Title: | Live imaging of ERK signaling dynamics in differentiating mouse embryonic stem cells |
Location: | England |
Open access status: | An open access version is available from UCL Discovery |
DOI: | 10.1242/dev.172940 |
Publisher version: | https://doi.org/10.1242/dev.172940 |
Language: | English |
Additional information: | This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions. |
Keywords: | ERK, Epigenetic inheritance, FRET, Nanog, Single cell, Stem cells |
UCL classification: | UCL UCL > Provost and Vice Provost Offices UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Lab for Molecular Cell Bio MRC-UCL |
URI: | https://discovery.ucl.ac.uk/id/eprint/10074531 |
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