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Polarized Two-Photon Absorption and Heterogeneous Fluorescence Dynamics in NAD(P)H

Blacker, TS; Nicolaou, N; Duchen, MR; Bain, AJ; (2019) Polarized Two-Photon Absorption and Heterogeneous Fluorescence Dynamics in NAD(P)H. The Journal of Physical Chemistry B 10.1021/acs.jpcb.9b01236. (In press). Green open access

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Abstract

Two-photon absorption (2PA) finds widespread application in biological systems, which frequently exhibit heterogeneous fluorescence decay dynamics corresponding to multiple species or environments. By combining polarised 2PA with time-resolved fluorescence intensity and anisotropy decay measurements, we show how the two-photon transition tensors for the components of a heterogeneous population can be separately determined, allowing structural differences between the two fluorescent states of the redox cofactor NAD(P)H to be identified. The results support the view that the two states correspond to alternate configurations of the nicotinamide ring, rather than folded and extended conformations of the entire molecule.

Type: Article
Title: Polarized Two-Photon Absorption and Heterogeneous Fluorescence Dynamics in NAD(P)H
Location: United States
Open access status: An open access version is available from UCL Discovery
DOI: 10.1021/acs.jpcb.9b01236
Publisher version: https://doi.org/10.1021/acs.jpcb.9b01236
Language: English
Additional information: Copyright © 2019 American Chemical Society. This is an open access article published under a Creative Commons Attribution (CC-BY) License (http://pubs.acs.org/page/policy/authorchoice_ccby_termsofuse.html), which permits unrestricted use, distribution and reproduction in any medium, provided the author and source are cited.
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Cell and Developmental Biology
UCL > Provost and Vice Provost Offices > UCL BEAMS
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences > Dept of Physics and Astronomy
URI: https://discovery.ucl.ac.uk/id/eprint/10073984
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