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CRB2 completes a fully expressed Crumbs complex in the Retinal Pigment Epithelium

Paniagua, AE; Herranz-Martin, S; Jimeno, D; Jimeno, AM; Lopez-Benito, S; Carlos Arevalo, J; Velasco, A; ... Lillo, C; + view all (2015) CRB2 completes a fully expressed Crumbs complex in the Retinal Pigment Epithelium. Scientific Reports , 5 , Article 14504. 10.1038/srep14504. Green open access

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Abstract

The CRB proteins CRB1, CRB2 and CRB3 are members of the cell polarity complex Crumbs in mammals that together with Scribble and Par complexes stablish the polarity of a variety of cell types. Although many members of the Crumbs complex proteins are expressed in the retinal pigment epithelium (RPE), and even though the mRNA of CRB2 has been detected in ARPE-19 cells and in the RPE/Choroid, to date no CRB protein has yet been found in this tissue. To investigate this possibility, we generated an antibody that specifically recognize the mouse CRB2 protein, and we demonstrate the expression of CRB2 in mouse RPE. Confocal analysis shows that CRB2 is restricted to the apicolateral membrane of RPE cells, and more precisely, in the tight junctions. Our study identified CRB2 as the member of the CRB protein family that is present together with the rest of the components of the Crumbs complex in the RPE apico-lateral cell membrane. Considering that the functions of CRB proteins are decisive in the establishment and maintenance of cell-cell junctions in several epithelial-derived cell types, we believe that these findings are a relevant starting point for unraveling the functions that CRB2 might perform in the RPE.

Type: Article
Title: CRB2 completes a fully expressed Crumbs complex in the Retinal Pigment Epithelium
Open access status: An open access version is available from UCL Discovery
DOI: 10.1038/srep14504
Publisher version: https://doi.org/10.1038/srep14504
Language: English
Additional information: © 2014 the Authors. This work is licensed under a Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/).
Keywords: Adherens junctions, Apicobasal polarity, Cellular neuroscience, Tight junctions
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Cell and Developmental Biology
URI: https://discovery.ucl.ac.uk/id/eprint/10073889
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