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Disease profiling by MALDI MS analysis of biofluids

Camuzeaux, S; Timms, JF; (2016) Disease profiling by MALDI MS analysis of biofluids. In: Cramer, R, (ed.) Advances in MALDI and Laser-Induced Soft Ionization Mass Spectrometry. (pp. 185-196). Springer: Cham, Switzerland. Green open access

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Abstract

There is an urgent need for accurate biomarkers of disease. The low-molecular weight proteome of blood serum or other biological fluids may be an ideal source of such biomarkers, although its analysis requires high-throughput strategies to enrich and quantify peptides and small proteins with biomarker potential. Herein, serum samples from cancer cases and controls are compared using a workflow of robotic reversed-phase extraction and clean-up, followed by automated MALDI MS spectral acquisition and analysis of the low-molecular weight peptidome. The aim of the presented methodology is to facilitate the discovery of candidate serum biomarkers of cancer using MALDI MS profiling, although the method is applicable to any comparative proteomic analysis of any biofluid.

Type: Book chapter
Title: Disease profiling by MALDI MS analysis of biofluids
ISBN-13: 9783319048185
Open access status: An open access version is available from UCL Discovery
DOI: 10.1007/978-3-319-04819-2_10
Publisher version: https://doi.org/10.1007/978-3-319-04819-2_10
Language: English
Additional information: This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions.
Keywords: MALDI MS, Proteomic profiling, MS profiling, Serum, Peptidome, LC-MS/MS, Blood, Biofluids, Sample handling
UCL classification: UCL
UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > UCL EGA Institute for Womens Health
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > UCL EGA Institute for Womens Health > Womens Cancer
URI: https://discovery.ucl.ac.uk/id/eprint/10073745
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