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Super-resolution fight club: assessment of 2D and 3D single-molecule localization microscopy software

Sage, D; Pham, T-A; Babcock, H; Lukes, T; Pengo, T; Chao, J; Velmurugan, R; ... Holden, S; + view all (2019) Super-resolution fight club: assessment of 2D and 3D single-molecule localization microscopy software. Nature Methods , 16 pp. 387-395. 10.1038/s41592-019-0364-4. Green open access

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Abstract

With the widespread uptake of two-dimensional (2D) and three-dimensional (3D) single-molecule localization microscopy (SMLM), a large set of different data analysis packages have been developed to generate super-resolution images. In a large community effort, we designed a competition to extensively characterize and rank the performance of 2D and 3D SMLM software packages. We generated realistic simulated datasets for popular imaging modalities-2D, astigmatic 3D, biplane 3D and double-helix 3D-and evaluated 36 participant packages against these data. This provides the first broad assessment of 3D SMLM software and provides a holistic view of how the latest 2D and 3D SMLM packages perform in realistic conditions. This resource allows researchers to identify optimal analytical software for their experiments, allows 3D SMLM software developers to benchmark new software against the current state of the art, and provides insight into the current limits of the field.

Type: Article
Title: Super-resolution fight club: assessment of 2D and 3D single-molecule localization microscopy software
Location: United States
Open access status: An open access version is available from UCL Discovery
DOI: 10.1038/s41592-019-0364-4
Publisher version: https://doi.org/10.1038/s41592-019-0364-4
Language: English
Additional information: This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions.
Keywords: Software, Super-resolution microscopy
UCL classification: UCL
UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Lab for Molecular Cell Bio MRC-UCL
URI: https://discovery.ucl.ac.uk/id/eprint/10073387
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