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α-actinin accounts for the bioactivity of actin preparations in inducing STAT target genes in Drosophila melanogaster

Gordon, O; Henry, CM; Srinivasan, N; Ahrens, S; Franz, A; Deddouche, S; Chakravarty, P; ... Reis E Sousa, C; + view all (2018) α-actinin accounts for the bioactivity of actin preparations in inducing STAT target genes in Drosophila melanogaster. eLife , 7 , Article e38636. 10.7554/eLife.38636. Green open access

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Abstract

Damage-associated molecular patterns (DAMPs) are molecules exposed or released by dead cells that trigger or modulate immunity and tissue repair. In vertebrates, the cytoskeletal component F-actin is a DAMP specifically recognised by DNGR-1, an innate immune receptor. Previously we suggested that actin is also a DAMP in Drosophila melanogaster by inducing STAT-dependent genes (Srinivasan et al., 2016). Here, we revise that conclusion and report that α-actinin is far more potent than actin at inducing the same STAT response and can be found in trace amounts in actin preparations. Recombinant expression of actin or α-actinin in bacteria demonstrated that only α-actinin could drive the expression of STAT target genes in Drosophila. The response to injected α-actinin required the same signalling cascade that we had identified in our previous work using actin preparations. Taken together, these data indicate that α-actinin rather than actin drives STAT activation when injected into Drosophila.

Type: Article
Title: α-actinin accounts for the bioactivity of actin preparations in inducing STAT target genes in Drosophila melanogaster
Location: England
Open access status: An open access version is available from UCL Discovery
DOI: 10.7554/eLife.38636
Publisher version: https://doi.org/10.7554/eLife.38636.001
Language: English
Additional information: © 2018, Gordon et al. This article is distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/).
Keywords: Research Advance, Immunology and Inflammation, Innate Immunity, Damage-Associated Molecular Pattern, Tissue Injury, Jak/Stat, Pathway, Damp, Sterile and Inflammation
UCL classification: UCL
UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Cell and Developmental Biology
URI: https://discovery.ucl.ac.uk/id/eprint/10073342
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