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Chapter 19 - Probing Ca2+ release mechanisms using sea urchin egg homogenates

Yuan, Y; Gunaratne, GS; Marchant, JS; Patel, S; (2019) Chapter 19 - Probing Ca2+ release mechanisms using sea urchin egg homogenates. Methods in Cell Biology , 151 pp. 445-458. 10.1016/bs.mcb.2018.10.007.

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Abstract

Sea urchin eggs have been extensively used to study Ca2+ release through intracellular Ca2+-permeable channels. Their amenability to homogenization yields a robust, cell-free preparation that was central to establishing the Ca2+ mobilizing actions of cyclic ADP-ribose and NAADP. Egg homogenates have continued to provide insight into the basic properties and pharmacology of intracellular Ca2+ release channels. In this chapter, we describe methods for the preparation of egg homogenates and monitoring Ca2+ release using fluorimetry and radiotracer flux.

Type: Article
Title: Chapter 19 - Probing Ca2+ release mechanisms using sea urchin egg homogenates
Location: United States
DOI: 10.1016/bs.mcb.2018.10.007
Publisher version: https://doi.org/10.1016/bs.mcb.2018.10.007
Language: English
Additional information: This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions.
Keywords: (45)Ca flux, Ca(2+), Cyclic-ADP ribose, Fluorimetry, Inositol trisphosphate, NAADP
UCL classification: UCL
UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Cell and Developmental Biology
URI: https://discovery.ucl.ac.uk/id/eprint/10073279
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