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Identification of Nuclear Receptor Targets by Chromatin Immunoprecipitation in Fatty Liver

Becares, N; Pineda-Torra, I; (2019) Identification of Nuclear Receptor Targets by Chromatin Immunoprecipitation in Fatty Liver. Lipid-Activated Nuclear Receptors , 1951 pp. 179-188. 10.1007/978-1-4939-9130-3_14. Green open access

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Abstract

Chromatin immunoprecipitation (ChIP) assays allow for the study of protein-DNA interactions in physiological contexts. Briefly, ChIPs consist of the purification and enrichment of a protein of interest together with its associated chromatin and its later identification. Hence, this technique proves to be particularly useful when assessing novel target genes for nuclear receptors. In the field of metabolic liver diseases, the validation of putative nuclear receptor targets is key for furthering the development of nuclear receptor modulators as therapeutic compounds. In this chapter, the protocol described has been optimized for ChIP on mouse fatty (steatotic) livers. Thanks to the use of a "two-step" (double) cross-linking method, this protocol can also be used for the study of other proteins with weaker interactions or that are present in large complexes, such as cofactors.

Type: Article
Title: Identification of Nuclear Receptor Targets by Chromatin Immunoprecipitation in Fatty Liver
Location: United States
Open access status: An open access version is available from UCL Discovery
DOI: 10.1007/978-1-4939-9130-3_14
Publisher version: https://doi.org/10.1007/978-1-4939-9130-3_14
Language: English
Additional information: This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions.
Keywords: Chromatin immunoprecipitation, Cross-linking, Fatty liver, Mouse model, Nuclear receptors
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Medicine
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Medicine > Experimental and Translational Medicine
URI: https://discovery.ucl.ac.uk/id/eprint/10073005
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