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Measuring Apoptotic Cell Engulfment (Efferocytosis) Efficiency

Gage, MC; (2019) Measuring Apoptotic Cell Engulfment (Efferocytosis) Efficiency. Methods in Molecular Biology , 1951 pp. 143-152. 10.1007/978-1-4939-9130-3_11. Green open access

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Abstract

Efferocytosis is the process of recognizing and removing dead and dying cells, performed by a variety of phagocytic cells including macrophages. It has recently been shown that liver X receptor (LXR) signaling in macrophages regulates the expression of important efferocytosis receptors, bridging and signaling molecules. Here we describe a sensitive yet robust efferocytosis assay, optimized to measure bone marrow-derived macrophage (BMDM) apoptotic cell engulfment capability. This assay can be applied to genetically or pharmacologically altered BMDMs.

Type: Article
Title: Measuring Apoptotic Cell Engulfment (Efferocytosis) Efficiency
Location: United States
Open access status: An open access version is available from UCL Discovery
DOI: 10.1007/978-1-4939-9130-3_11
Publisher version: https://doi.org/10.1007/978-1-4939-9130-3_11
Language: English
Additional information: This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions.
Keywords: Nuclear receptors, efferocytosis, apoptosis, macrophages, BMDM, Jurkats, engulfment.
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Medicine
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Medicine > Experimental and Translational Medicine
URI: https://discovery.ucl.ac.uk/id/eprint/10072165
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