Barroeta Seijas, A;
Rodney Mijares, M;
Ochoa, M;
Martinez, GP;
Suarez, AI;
Santi Compagnone, R;
Chirinos, P;
... De Sanctis, JB; + view all
(2013)
Cytotoxic effects of Fisturalin-3 and 11-Deoxyfisturalin-3 on Jurkat and U937 cell lines.
Biomedical papers of the Medical Faculty of the University Palacky, Olomouc, Czechoslovakia
, 157
(3)
pp. 222-226.
10.5507/bp.2012.089.
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Abstract
Background. Fisturalines are bromotyrosine compounds isolated from marine sponges. Previous studies have shown antineoplasic, antiviral and antibacterial effects in Vitro; however, the possible effects of these compounds in hematologic malignancies have not been assessed. Methods. In the present study, the antiproliferative and pro apoptotic effects of Fistularin-3 (F) and 11-Deoxyfistularin-3 (DF) were assessed using the MTT method and annexin V/propidium iodide by flow cytometry using the cell lines: Jurkat E6.1 and U937. In addition, the cell cycle was assessed by flow cytometry. Results. Inhibition of the proliferative response was concentration and time dependent. The IC50 of F was 7.39 and 8.10 M for Jurkat E6.1 and U937 respectively. At 24 and 48 h, in the U937 cell line, but not in the Jurkat cell line, both compounds induced up to 35% annexin V increase. Necrosis was not observed in any case. Compound F induced, in both cell lines, a decrease in the number of cells in the S phase and increase in the G0/G1 phase. In the Jurkat cell line only, there was an increase in the number of cells in the G2/M phase. Compound DF was not as effective as F. Conclusions. F is more active than DF in repressing the cell cycle and inducing apoptosis. Both compounds are potentially useful in the development of new drugs to treat hematologic malignancies.
| Type: | Article |
|---|---|
| Title: | Cytotoxic effects of Fisturalin-3 and 11-Deoxyfisturalin-3 on Jurkat and U937 cell lines |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.5507/bp.2012.089 |
| Publisher version: | https://doi.org/10.5507/bp.2012.089 |
| Language: | English |
| Additional information: | This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
| Keywords: | Fistularin-3, 11-Deoxyfistularin-3, leukemia, cancer, bromotyrosine, quercetin, Aplysina fistularis |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > UCL GOS Institute of Child Health UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > UCL GOS Institute of Child Health > Infection, Immunity and Inflammation Dept |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10072106 |
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