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A novel, lineage-primed prestalk cell subtype involved in the morphogenesis of D-discoideum

Kuwana, S; Senoo, H; Sawai, S; Fukuzawa, M; (2016) A novel, lineage-primed prestalk cell subtype involved in the morphogenesis of D-discoideum. Developmental Biology , 416 (2) pp. 286-299. 10.1016/j.ydbio.2016.06.032. Green open access

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Abstract

Dictyostelium morphogenesis requires the tip, which acts as an organizer and conducts orchestrated cell movement and cell differentiation. At the slug stage the tip region contains prestalk A (pstA) cells, which are usually recognized by their expression of reporter constructs that utilize a fragment of the promoter of the ecmA gene. Here, using the promoter region of the o-methyl transferase 12 gene (omt12) to drive reporter expression, we demonstrate the presence, also within the pstA region, of a novel prestalk cell subtype: the pstVA cells. Surprisingly, a sub-population of the vegetative cells express a pstVA: GFP marker and, sort out to the tip, both when developing alone and when co-developed with an excess of unmarked cells. The development of such a purified GFP-marked population is greatly accelerated: by precocious cell aggregation and tip formation with accompanying precocious elevation of developmental gene transcription. We therefore suggest that the tip contains at least two prestalk cell subtypes: the developmentally-specified pstA cells and the lineage-primed pstVA cells. It is presumably the pstVA cells that play the dominant role in morphogenesis during the earlier stages of development. The basis for the lineage priming is, however, unclear because we can find no correlation between pstVA differentiation and nutrient status during growth or cell cycle position at the time of starvation, the two known determinants of probable cell fate.

Type: Article
Title: A novel, lineage-primed prestalk cell subtype involved in the morphogenesis of D-discoideum
Open access status: An open access version is available from UCL Discovery
DOI: 10.1016/j.ydbio.2016.06.032
Publisher version: https://doi.org/10.1016/j.ydbio.2016.06.032
Language: English
Additional information: This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions.
Keywords: morphogenesis, organizer, Dictyostelium, prestalk cell, lineage-priming, heterogeneity
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Genetics, Evolution and Environment
URI: https://discovery.ucl.ac.uk/id/eprint/10071812
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