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Acceptability and feasibility study of patient-specific ‘tumouroids’ as personalised treatment screening tools: protocol for prospective tissue and data collection of participants with confirmed or suspected renal cell carcinoma

Tran, M; Neves, J; Stamati, K; Redondo, P; Cope, A; Brew-Graves, C; Williams, N; ... Emberton, M; + view all (2019) Acceptability and feasibility study of patient-specific ‘tumouroids’ as personalised treatment screening tools: protocol for prospective tissue and data collection of participants with confirmed or suspected renal cell carcinoma. International Journal of Surgery Protocols , 14 pp. 24-29. 10.1016/j.isjp.2019.03.019. Green open access

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Abstract

Introduction: ‘Personalised medicine’ aims to tailor interventions to the individual, and has become one of the fastest growing areas of cancer research. One of these approaches is to harvest cancer cells from patients and grow them in the laboratory, which can then be subjected to treatments and the response assessed. We have developed a 3D tumour model with a complex protein matrix that mimics the tumour stroma, cell to cell and cell-matrix interactions seen in vivo, called a tumouroid. In this study, we test the acceptability and feasibility of using this model to establish patient-derived tumouroids. Methods and analysis This is a first in-human study using prospective tissue and data collection of adult participants with confirmed or suspected renal cell carcinoma. The goals of the study are to assess patient acceptability to the use of patient-derived tumour models for future treatment decisions, and to assess the feasibility of generating patient-specific renal cancer tumouroids that can be challenged with drugs. These goals will be realised through the collection of tumour samples (expected n=10), participant-completed questionnaires (expected n=10), and in-depth semi-structured interviews with patients (expected n=5). Collected multiregional tumour samples will be dissociated to isolate primary cells which are then expanded in vitro and incorporated into tumouroids. Drug challenge will ensue and the response will be categorised into “responder”, “weak responder”, and “non-responder”. Statistical analysis will be descriptive. Ethics and dissemination: The study has ethical approval (REC reference 17/LO/1744). Findings will be made available to patients, clinicians, funders, and the National Health Service (NHS) through presentations at national and international meetings, peer-reviewed publications, social media and patient support groups. Trial Registration: Registered on ClinicalTrials.gov (NCT03300102).

Type: Article
Title: Acceptability and feasibility study of patient-specific ‘tumouroids’ as personalised treatment screening tools: protocol for prospective tissue and data collection of participants with confirmed or suspected renal cell carcinoma
Open access status: An open access version is available from UCL Discovery
DOI: 10.1016/j.isjp.2019.03.019
Publisher version: https://doi.org/10.1016/j.isjp.2019.03.019
Language: English
Additional information: Copyright © 2019 IJS Publishing Group Limited. Published by Elsevier Ltd. Open Access. This article is distributed under the terms of the Creative Attribution-NonCommercial -NoDerivatives 4.0 International (CC BY-NC-ND 4.0) license which permits any use, reproduction and distribution of the work in any medium or format without further permission provided the original work is attributed.
Keywords: Renal cell carcinoma, precision medicine, in vitro techniques, drug screening assays, personalised cancer treatment, 3D in vitro cancer models
UCL classification: UCL
UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Medicine
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Medicine > Metabolism and Experi Therapeutics
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Surgery and Interventional Sci
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Surgery and Interventional Sci > Department of Ortho and MSK Science
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Surgery and Interventional Sci > Department of Surgical Biotechnology
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Surgery and Interventional Sci > Department of Targeted Intervention
UCL > Provost and Vice Provost Offices > VP: Health
UCL > Provost and Vice Provost Offices > VP: Health > Translational Research Office
URI: https://discovery.ucl.ac.uk/id/eprint/10071596
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