UCL Discovery
UCL home » Library Services » Electronic resources » UCL Discovery

Pseudomonas aeruginosa biofilm is a potent inducer of phagocyte hyperinflammation

Ciszek-Lenda, M; Strus, M; Walczewska, M; Majka, G; Machul-Żwirbla, A; Mikołajczyk, D; Górska, S; ... Marcinkiewicz, J; + view all (2019) Pseudomonas aeruginosa biofilm is a potent inducer of phagocyte hyperinflammation. Inflammation Research 10.1007/s00011-019-01227-x. (In press). Green open access

[thumbnail of Chain_Pseudomonas aeruginosa biofilm is a potent inducer of phagocyte hyperinflammation_Proof.pdf]
Preview
Text
Chain_Pseudomonas aeruginosa biofilm is a potent inducer of phagocyte hyperinflammation_Proof.pdf

Download (5MB) | Preview

Abstract

OBJECTIVE: Pseudomonas aeruginosa effectively facilitate resistance to phagocyte killing by biofilm formation. However, the cross talk between biofilm components and phagocytes is still unclear. We hypothesize that a biofilm provides a concentrated extracellular source of LPS, DNA and exopolysaccharides (EPS), which polarize neighbouring phagocytes into an adverse hyperinflammatory state of activation. METHODS: We measured the release of a panel of mediators produced in vitro by murine neutrophils and macrophages exposed to various biofilm components of P. aeruginosa cultures. RESULTS: We found that conditioned media from a high biofilm-producing strain of P. aeruginosa, PAR5, accumulated high concentrations of extracellular bacterial LPS, DNA and EPS by 72 h. These conditioned media induced phagocytes to release a hyperinflammatory pattern of mediators, with enhanced levels of TNF-α, IL-6, IL12p40, PGE2 and NO. Moreover, the phagocytes also upregulated COX-2 and iNOS with no influence on the expression of arginase-1. CONCLUSIONS: Phagocytes exposed to biofilm microenvironment, called by us biofilm-associated neutrophils/macrophages (BANs/BAMs), display secretory properties similar to that of N1/M1-type phagocytes. These results suggest that in vivo high concentrations of LPS and DNA, trapped in biofilm by EPS, might convert infiltrating phagocytes into cells responsible for tissue injury without direct contact with bacteria and phagocytosis.

Type: Article
Title: Pseudomonas aeruginosa biofilm is a potent inducer of phagocyte hyperinflammation
Location: Switzerland
Open access status: An open access version is available from UCL Discovery
DOI: 10.1007/s00011-019-01227-x
Publisher version: http://dx.doi.org/10.1007/s00011-019-01227-x
Language: English
Additional information: © The Author(s) 2019. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/).
Keywords: Biofilm, DNA, Hyperinflammation, LPS, Neutrophils, P. aeruginosa
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Infection and Immunity
URI: https://discovery.ucl.ac.uk/id/eprint/10071110
Downloads since deposit
119Downloads
Download activity - last month
Download activity - last 12 months
Downloads by country - last 12 months

Archive Staff Only

View Item View Item