Pereira, PM;
Jacobs, C;
Grove, J;
(2019)
Investigating Hepatitis C Virus Infection Using Super-Resolution Microscopy.
Hepatitis C Virus Protocols: Methods in Molecular Biology
, 1911
pp. 247-261.
10.1007/978-1-4939-8976-8_17.
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Abstract
Super-resolution microscopy (SRM) can provide a window on the nanoscale events of virus replication. Here we describe a protocol for imaging hepatitis C virus-infected cells using localization SRM. We provide details on sample preparation, immunostaining, data collection, and super-resolution image reconstruction. We have made all efforts to generalize the protocol to make it accessible to all budding super-resolution microscopists.
| Type: | Article |
|---|---|
| Title: | Investigating Hepatitis C Virus Infection Using Super-Resolution Microscopy |
| Location: | United States |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1007/978-1-4939-8976-8_17 |
| Publisher version: | http://doi.org/10.1007/978-1-4939-8976-8_17 |
| Language: | English |
| Additional information: | This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions. |
| Keywords: | Hepatitis, Imaging, Microscopy, Super-resolution, Virus |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Infection and Immunity |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10070969 |
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