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Cryo-EM structure of the bacteria-killing type IV secretion system core complex from Xanthomonas citri

Sgro, GG; Costa, TRD; Cenens, W; Souza, DP; Cassago, A; de Oliveira, LC; Salinas, RK; ... Waksman, G; + view all (2018) Cryo-EM structure of the bacteria-killing type IV secretion system core complex from Xanthomonas citri. Nature Microbiology , 3 (12) pp. 1429-1440. 10.1038/s41564-018-0262-z. Green open access

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Abstract

Type IV secretion (T4S) systems form the most common and versatile class of secretion systems in bacteria, capable of injecting both proteins and DNAs into host cells. T4S systems are typically composed of 12 components that form 2 major assemblies: the inner membrane complex embedded in the inner membrane and the core complex embedded in both the inner and outer membranes. Here we present the 3.3 Å-resolution cryo-electron microscopy model of the T4S system core complex from Xanthomonas citri, a phytopathogen that utilizes this system to kill bacterial competitors. An extensive mutational investigation was performed to probe the vast network of protein–protein interactions in this 1.13-MDa assembly. This structure expands our knowledge of the molecular details of T4S system organization, assembly and evolution.

Type: Article
Title: Cryo-EM structure of the bacteria-killing type IV secretion system core complex from Xanthomonas citri
Open access status: An open access version is available from UCL Discovery
DOI: 10.1038/s41564-018-0262-z
Publisher version: https://doi.org/10.1038/s41564-018-0262-z
Language: English
Additional information: This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions.
UCL classification: UCL
UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Structural and Molecular Biology
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Lab for Molecular Cell Bio MRC-UCL
URI: https://discovery.ucl.ac.uk/id/eprint/10070938
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