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VIH2 Regulates the Synthesis of Inositol Pyrophosphate InsP₈ and Jasmonate-Dependent Defenses in Arabidopsis

Laha, D; Johnen, P; Azevedo, C; Dynowski, M; Weiss, M; Capolicchio, S; Mao, H; ... Schaaf, G; + view all (2015) VIH2 Regulates the Synthesis of Inositol Pyrophosphate InsP₈ and Jasmonate-Dependent Defenses in Arabidopsis. The Plant Cell , 27 (4) pp. 1082-1097. Green open access

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Abstract

Diphosphorylated inositol polyphosphates, also referred to as inositol pyrophosphates, are important signaling molecules that regulate critical cellular activities in many eukaryotic organisms, such as membrane trafficking, telomere maintenance, ribosome biogenesis, and apoptosis. In mammals and fungi, two distinct classes of inositol phosphate kinases mediate biosynthesis of inositol pyrophosphates: Kcs1/IP6K- and Vip1/PPIP5K-like proteins. Here, we report that PPIP5K homologs are widely distributed in plants and that Arabidopsis thaliana VIH1 and VIH2 are functional PPIP5K enzymes. We show a specific induction of inositol pyrophosphate InsP8 by jasmonate and demonstrate that steady state and jasmonate-induced pools of InsP8 in Arabidopsis seedlings depend on VIH2. We identify a role of VIH2 in regulating jasmonate perception and plant defenses against herbivorous insects and necrotrophic fungi. In silico docking experiments and radioligand binding-based reconstitution assays show high-affinity binding of inositol pyrophosphates to the F-box protein COI1-JAZ jasmonate coreceptor complex and suggest that coincidence detection of jasmonate and InsP8 by COI1-JAZ is a critical component in jasmonate-regulated defenses.

Type: Article
Title: VIH2 Regulates the Synthesis of Inositol Pyrophosphate InsP₈ and Jasmonate-Dependent Defenses in Arabidopsis
Open access status: An open access version is available from UCL Discovery
Publisher version: https://doi.org/10.1105/tpc.114.135160
Language: English
Additional information: This version is the version of record. For information on re-use, please refer to the publisher’s terms and conditions.
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Lab for Molecular Cell Bio MRC-UCL
URI: https://discovery.ucl.ac.uk/id/eprint/10070866
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