Maldonado, SL;
Krull, J;
Rasch, D;
Panjan, P;
Sesay, AM;
Marques, MPC;
Szita, N;
(2019)
Application of a multiphase microreactor chemostat for the determination of reaction kinetics of Staphylococcus carnosus.
Bioprocess and Biosystems Engineering
, 42
pp. 953-961.
10.1007/s00449-019-02095-9.
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Abstract
Bioreactors at the microliter scale offer a promising approach to accelerate bioprocess development. Advantages of such microbioreactors include a reduction in the use of expensive reagents. In this study, a chemostat operation mode of a cuvette-based microbubble column bioreactor made of polystyrene (working volume of 550 µL) was demonstrated. Aeration occurs through a nozzle (Ø ≤ 100 µm) and supports submerged whole-cell cultivation of Staphylococcus carnosus. Stationary concentrations of biomass and glucose were determined in the dilution rate regime ranging from 0.12 to 0.80 1/h with a glucose feed concentration of 1 g/L. For the first time, reaction kinetics of S. carnosus were estimated from data obtained from continuous cultivation. The maximal specific growth rate (µmax = 0.824 1/h), Monod constant (KS = 34 × 10- 3gS/L), substrate-related biomass yield coefficient (YX/S = 0.315 gCDW/gS), and maintenance coefficient (mS = 0.0035 gS/(gCDW·h)) were determined. These parameters are now available for further studies in the field of synthetic biology.
| Type: | Article |
|---|---|
| Title: | Application of a multiphase microreactor chemostat for the determination of reaction kinetics of Staphylococcus carnosus |
| Location: | Germany |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1007/s00449-019-02095-9 |
| Publisher version: | https://doi.org/10.1007/s00449-019-02095-9 |
| Language: | English |
| Additional information: | This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions. |
| Keywords: | Chemostat, Microbioreactor, Microbubble column bioreactor, Reaction kinetics, Staphylococcus carnosus |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > UCL BEAMS UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Biochemical Engineering |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10070795 |
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