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Application of a multiphase microreactor chemostat for the determination of reaction kinetics of Staphylococcus carnosus

Maldonado, SL; Krull, J; Rasch, D; Panjan, P; Sesay, AM; Marques, MPC; Szita, N; (2019) Application of a multiphase microreactor chemostat for the determination of reaction kinetics of Staphylococcus carnosus. Bioprocess and Biosystems Engineering , 42 pp. 953-961. 10.1007/s00449-019-02095-9. Green open access

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Abstract

Bioreactors at the microliter scale offer a promising approach to accelerate bioprocess development. Advantages of such microbioreactors include a reduction in the use of expensive reagents. In this study, a chemostat operation mode of a cuvette-based microbubble column bioreactor made of polystyrene (working volume of 550 µL) was demonstrated. Aeration occurs through a nozzle (Ø ≤ 100 µm) and supports submerged whole-cell cultivation of Staphylococcus carnosus. Stationary concentrations of biomass and glucose were determined in the dilution rate regime ranging from 0.12 to 0.80 1/h with a glucose feed concentration of 1 g/L. For the first time, reaction kinetics of S. carnosus were estimated from data obtained from continuous cultivation. The maximal specific growth rate (µmax = 0.824 1/h), Monod constant (KS = 34 × 10- 3gS/L), substrate-related biomass yield coefficient (YX/S = 0.315 gCDW/gS), and maintenance coefficient (mS = 0.0035 gS/(gCDW·h)) were determined. These parameters are now available for further studies in the field of synthetic biology.

Type: Article
Title: Application of a multiphase microreactor chemostat for the determination of reaction kinetics of Staphylococcus carnosus
Location: Germany
Open access status: An open access version is available from UCL Discovery
DOI: 10.1007/s00449-019-02095-9
Publisher version: https://doi.org/10.1007/s00449-019-02095-9
Language: English
Additional information: This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions.
Keywords: Chemostat, Microbioreactor, Microbubble column bioreactor, Reaction kinetics, Staphylococcus carnosus
UCL classification: UCL
UCL > Provost and Vice Provost Offices > UCL BEAMS
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Biochemical Engineering
URI: https://discovery.ucl.ac.uk/id/eprint/10070795
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