Zhou, J;
Ding, Z-M;
Hardiman, PJ;
(2018)
Understanding the Role of Gui-Zhi-Fu-Ling-Capsules (Chinese Medicine) for Treatment of Endometriosis in the Rat Model: Using NMR Based Metabolomics.
Evidence-Based Complementary and Alternative Medicine
, 2018
, Article 9864963. 10.1155/2018/9864963.
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Abstract
Te objective of this study is to identify the changes of metabolites in the rat endometriosis models treated with Gui-Zhi-Fu-Lingcapsules (GZFLC), a classic Chinese medicinal formula, and to explore the efects of GZFLC on the serum levels of transforming growth factor-�1 (TGF-�1) and the mRNA expression levels of vascular endothelial growth factor (VEGF) and glucose transporter 4 (GLUT-4) in the endometriotic tissues. Forty female Wistar rats were randomly divided into the sham-operation group (Normal group), Model group, Danazol group, and GZFLC group.Te serum levels of TGF-�1 were measured using enzyme-linked immunesorbent assay (ELISA). Te mRNA expression levels of VEGF and GLUT-4 in the endometriotic tissue of the rat endometriosis models were measured using real-time quantitative PCR. Te metabolites in urine were detected by 1 H NMR method. Eight identifed metabolites of the NMR resonance were involved in the glycolysis metabolism. Among the 8 metabolites, Lactate, Acetate, TMA, and Formate were downregulated with GZFLC. Citrate, TMAO, Taurine, and Hippurate were unregulated with GZFLC. Te serum levels of TGF-�1 in the Danazol and GZFLC groups were signifcantly higher than those of Normal group and signifcantly lower than the Model group. GZFLC treatment signifcantly decreased the GLUT-4 and VEGF mRNA expression levels in the endometriotic tissues of the endometriosis rats (P < 0.05). GZFLC signifcantly decreased the GLUT-4 mRNA expression levels in rats of GZFLC group compared with Danazol group. It is through regulating the metabolites changes of glycolysis or gluconeogenesis that GZFLC signifcantly afected the expression levels of TGF-�1, GLUT-4, and VEGF of the model rats with endometriosis.
| Type: | Article |
|---|---|
| Title: | Understanding the Role of Gui-Zhi-Fu-Ling-Capsules (Chinese Medicine) for Treatment of Endometriosis in the Rat Model: Using NMR Based Metabolomics. |
| Location: | United States |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1155/2018/9864963 |
| Publisher version: | https://doi.org/10.1155/2018/9864963 |
| Language: | English |
| Additional information: | This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. https://creativecommons.org/licenses/by/4.0/ |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > UCL EGA Institute for Womens Health UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > UCL EGA Institute for Womens Health > Reproductive Health |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10070586 |
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