Reynolds, CJ;
Chong, DLW;
Li, Y;
Black, SL;
Cutler, A;
Webster, Z;
Manji, J;
... Boyton, RJ; + view all
(2019)
Bioluminescent Reporting of In Vivo IFN-γ Immune Responses during Infection and Autoimmunity.
Journal of Immunology
, 202
(6)
, Article 1647. 10.4049/jimmunol.1801453.
Preview |
Text
jimmunol.1801453.full.pdf - Published Version Download (1MB) | Preview |
Abstract
IFN-γ is a key cytokine of innate and adaptive immunity. It is important to understand temporal changes in IFN-γ production and how these changes relate to the role of IFN-γ in diverse models of infectious and autoimmune disease, making the ability to monitor and track IFN-γ production in vivo of a substantial benefit. IFN-γ ELISPOTs have been a central methodology to measure T cell immunity for many years. In this study, we add the capacity to analyze IFN-γ responses with high sensitivity and specificity, longitudinally, in vitro and in vivo. This allows the refinement of experimental protocols because immunity can be tracked in real-time through a longitudinal approach. We have generated a novel murine IFN-γ reporter transgenic model that allows IFN-γ production to be visualized and quantified in vitro and in vivo as bioluminescence using an imaging system. At baseline, in the absence of an inflammatory stimulus, IFN-γ signal from lymphoid tissue is detectable in vivo. Reporter transgenics are used in this study to track the IFN-γ response to Pseudomonas aeruginosa infection in the lung over time in vivo. The longitudinal development of the adaptive T cell immunity following immunization with Ag is identified from day 7 in vivo. Finally, we show that we are able to use this reporter transgenic to follow the onset of autoimmune T cell activation after regulatory T cell depletion in an established model of systemic autoimmunity. This IFN-γ reporter transgenic, termed "Gammaglow," offers a valuable new modality for tracking IFN-γ immunity, noninvasively and longitudinally over time.
| Type: | Article |
|---|---|
| Title: | Bioluminescent Reporting of In Vivo IFN-γ Immune Responses during Infection and Autoimmunity |
| Location: | United States |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.4049/jimmunol.1801453 |
| Publisher version: | https://doi.org/10.4049/jimmunol.1801453 |
| Language: | English |
| Additional information: | This work is licensed under a CC BY 4.0 Unported license. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit: https://creativecommons.org/licenses/by/4.0/ |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Medicine UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Medicine > Nephrology |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10070008 |
Archive Staff Only
 |
View Item |
