Saeed, S;
Lau, C-I;
Tremp, AZ;
Crompton, T;
Dessens, JT;
(2019)
Dysregulated gene expression in oocysts of Plasmodium berghei LAP mutants.
Molecular and Biochemical Parasitology
, 229
10.1016/j.molbiopara.2019.02.001.
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Abstract
Malaria parasite oocysts generate sporozoites by a process termed sporogony. Essential for successful sporogony of Plasmodium berghei in Anopheles stephensi mosquitoes is a complex of six LCCL lectin domain adhesive-like proteins (LAPs). LAP null mutant oocysts undergo growth and mitosis but fail to form sporozoites. At a cytological level, LAP null mutant oocyst development is indistinguishable from its wildtype counterparts for the first week, supporting the hypothesis that LAP null mutant oocysts develop normally before cytokinesis. We show here that LAP1 null mutant oocysts display highly reduced expression of sporozoite proteins and their transcription factors. Our findings indicate that events leading up to the cytokinesis defect in LAP null mutants occur early in oocyst development.
| Type: | Article |
|---|---|
| Title: | Dysregulated gene expression in oocysts of Plasmodium berghei LAP mutants |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1016/j.molbiopara.2019.02.001 |
| Publisher version: | https://doi.org/10.1016/j.molbiopara.2019.02.001 |
| Language: | English |
| Additional information: | © 2019 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY license (http://creativecommons.org/licenses/BY/4.0/). |
| Keywords: | LCCL, Malaria transmission, Oocyst, Plasmodium berghei, Sporogony |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > UCL GOS Institute of Child Health UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > UCL GOS Institute of Child Health > Infection, Immunity and Inflammation Dept |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10069000 |
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