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Peptidomic Analysis of Cartilage and Subchondral bone in OA patients

Gatenholm, B; Gobom, J; Skillbäck, T; Blennow, K; Zetterberg, H; Brittberg, M; (2019) Peptidomic Analysis of Cartilage and Subchondral bone in OA patients. European Journal of Clinical Investigation , 49 (5) , Article e13082. 10.1111/eci.13082. Green open access

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Abstract

BACKGROUND: The objective of this study was to develop a method for directly analyzing osteochondral samples straight out of the operating room without cell culturing, thereby enabling identification of potential peptide biomarkers to better understand the mechanisms involved in the development of osteoarthritis and pain. MATERIAL AND METHODS: Osteochondral plugs from wounded and macroscopically non-wounded zones of the femur condyle were collected from 6 patients with manifest osteoarthritis (OA) undergoing total knee arthroplasty (TKA). The samples were demineralized and supernatant was collected and isotopically marked with Tandem Mass Tag (TMT) labeling and analyzed using liquid chromatography coupled with tandem mass spectrometry LC-MS/MS. RESULTS: Using peptidomics, 6292 endogenous peptides were identified. Five hundred sixty six peptides (8 identified endogenous peptides) differed significantly (p-value 0.10) from wounded zones compared to non-wounded zones. CONCLUSION: This pilot study shows promising results for enabling peptidomic analysis of cartilage and bone straight out of the operating room. With further refinement, peptidomics can potentially become a diagnostic tool for OA, and improve the knowledge of disease progression and genesis of pain. This article is protected by copyright. All rights reserved.

Type: Article
Title: Peptidomic Analysis of Cartilage and Subchondral bone in OA patients
Location: England
Open access status: An open access version is available from UCL Discovery
DOI: 10.1111/eci.13082
Publisher version: https://doi.org/10.1111/eci.13082
Language: English
Additional information: This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions.
Keywords: Cartilage, Mass spectrometry, Neuropeptides, Osteoarthritis, Pain
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > UCL Queen Square Institute of Neurology
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > UCL Queen Square Institute of Neurology > Neurodegenerative Diseases
URI: https://discovery.ucl.ac.uk/id/eprint/10068879
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