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Prominin-1 controls stem cell activation by orchestrating ciliary dynamics

Singer, D; Thamm, K; Zhuang, H; Karbanová, J; Gao, Y; Walker, JV; Jin, H; ... Hu, B; + view all (2019) Prominin-1 controls stem cell activation by orchestrating ciliary dynamics. The EMBO Journal , 38 (1) , Article e99845. 10.15252/embj.201899845. Green open access

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Abstract

Proper temporal and spatial activation of stem cells relies on highly coordinated cell signaling. The primary cilium is the sensory organelle that is responsible for transmitting extracellular signals into a cell. Primary cilium size, architecture, and assembly-disassembly dynamics are under rigid cell cycle-dependent control. Using mouse incisor tooth epithelia as a model, we show that ciliary dynamics in stem cells require the proper functions of a cholesterol-binding membrane glycoprotein, Prominin-1 (Prom1/CD133), which controls sequential recruitment of ciliary membrane components, histone deacetylase, and transcription factors. Nuclear translocation of Prom1 and these molecules is particularly evident in transit amplifying cells, the immediate derivatives of stem cells. The absence of Prom1 impairs ciliary dynamics and abolishes the growth stimulation effects of sonic hedgehog (SHH) treatment, resulting in the disruption of stem cell quiescence maintenance and activation. We propose that Prom1 is a key regulator ensuring appropriate response of stem cells to extracellular signals, with important implications for development, regeneration, and diseases.

Type: Article
Title: Prominin-1 controls stem cell activation by orchestrating ciliary dynamics
Open access status: An open access version is available from UCL Discovery
DOI: 10.15252/embj.201899845
Publisher version: https://doi.org/10.15252/embj.201899845
Language: English
Additional information: This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions.
Keywords: CD133; cilia; sonic hedgehog; stem cells; tooth; Cell Adhesion, Polarity & Cytoskeleton; Development & Differentiation; Stem Cells
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Cancer Institute
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Cancer Institute > Research Department of Cancer Bio
URI: https://discovery.ucl.ac.uk/id/eprint/10065174
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