UCL Discovery
UCL home » Library Services » Electronic resources » UCL Discovery

The synthesis of recombinant membrane proteins in yeast for structural studies

Routledge, SJ; Mikaliunaite, L; Patel, A; Clare, M; Cartwright, SP; Bawa, Z; Wilks, MDB; ... Bill, RM; + view all (2016) The synthesis of recombinant membrane proteins in yeast for structural studies. Methods , 95 pp. 26-37. 10.1016/j.ymeth.2015.09.027. Green open access

[thumbnail of Routledge_1-s2.0-S1046202315301134-main.pdf]
Preview
Text
Routledge_1-s2.0-S1046202315301134-main.pdf - Published Version

Download (1MB) | Preview

Abstract

Historically, recombinant membrane protein production has been a major challenge meaning that many fewer membrane protein structures have been published than those of soluble proteins. However, there has been a recent, almost exponential increase in the number of membrane protein structures being deposited in the Protein Data Bank. This suggests that empirical methods are now available that can ensure the required protein supply for these difficult targets. This review focuses on methods that are available for protein production in yeast, which is an important source of recombinant eukaryotic membrane proteins. We provide an overview of approaches to optimize the expression plasmid, host cell and culture conditions, as well as the extraction and purification of functional protein for crystallization trials in preparation for structural studies.

Type: Article
Title: The synthesis of recombinant membrane proteins in yeast for structural studies
Open access status: An open access version is available from UCL Discovery
DOI: 10.1016/j.ymeth.2015.09.027
Publisher version: https://doi.org/10.1016/j.ymeth.2015.09.027
Language: English
Additional information: © 2015 The Authors. This is an open access article under the CC BY license (http:// creativecommons.org/licenses/by/4.0/).
Keywords: Membrane proteins, Yeast, Recombinant, Expression plasmid vector, Promoter, SMALP, Cholesterol
UCL classification: UCL
UCL > Provost and Vice Provost Offices > UCL BEAMS
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Biochemical Engineering
URI: https://discovery.ucl.ac.uk/id/eprint/10064905
Downloads since deposit
89Downloads
Download activity - last month
Download activity - last 12 months
Downloads by country - last 12 months

Archive Staff Only

View Item View Item