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RNA Processing Dysfunction in TDP-ALS

Sivakumar, Prasanth; (2018) RNA Processing Dysfunction in TDP-ALS. Doctoral thesis (Ph.D), UCL (University College London). Green open access

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Abstract

Background: RNA processing dysfunction has been implicated in the pathology of the neurodegenerative disease amyotrophic lateral sclerosis (ALS), not only due to causative TDP-43 disease mutations (TDP-ALS), but further the characteristic mislocalisation of crucial RNA-binding protein TDP-43 regardless of genetic background. This implicates the importance of investigating the widespread TDP-43 dysfunction-mediated changes in RNA processing, and the need to identify differential gene expression and alternative splicing changes that may underlie neurodegenerative disease. Methods: Two mouse models of TDP-43 were investigated, each containing a single substitution within the coding region of the Tardbp gene: one mutation in the RNA binding domain (RBD), the other in the low complexity domain hotspot region for ALS-causative mutations. RNA sequencing was used to examine resulting differential gene expression and alternative splicing events, and integrated iCLIP analysis highlighted changes in RNA binding patterns. Results and discussion: We identified severe molecular dysregulation in both models. The RBD mutant displayed dose-dependent preferential exon inclusion, including the retention of cryptic exons. Alongside this was downregulation of long intron-containing genes, typically related to neuronal activity. These features, alongside other RNA processing changes, were similar to those resulting from TDP-43 loss, and thus indicated the RBD mutation to cause TDP-43 loss of function. Contrastingly, we found that the LCD mutation caused greater levels of exon skipping, including novel gain of TDP-43 function in splicing which resulted in mutant-specific ‘skiptic’ transcripts. A subset of these was then demonstrated to be conserved in a TDP-ALS mouse model, and further in human TDP-ALS patient settings. Importantly, this gain of function mouse model developed a neurodegenerative, ALS-like phenotype. This indicated that TDP-43 gain of function, even in the absence of loss of function effects, is sufficient to cause a neurodegenerative disease phenotype.

Type: Thesis (Doctoral)
Qualification: Ph.D
Title: RNA Processing Dysfunction in TDP-ALS
Event: UCL (University College London)
Open access status: An open access version is available from UCL Discovery
Language: English
Additional information: Copyright © The Author 2019. Original content in this thesis is licensed under the terms of the Creative Commons Attribution 4.0 International (CC BY 4.0) Licence (https://creativecommons.org/licenses/by/4.0/). Any third-party copyright material present remains the property of its respective owner(s) and is licensed under its existing terms. Access may initially be restricted at the author’s request.
UCL classification: UCL
UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > UCL Queen Square Institute of Neurology
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > UCL Queen Square Institute of Neurology > Department of Neuromuscular Diseases
URI: https://discovery.ucl.ac.uk/id/eprint/10064816
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