Papetti, A;
Signoretto, C;
Spratt, DA;
Pratten, J;
Lingstrom, P;
Zaura, E;
Ofek, I;
... Gazzani, G; + view all
(2018)
Components in Lentinus edodes mushroom with anti-biofilm activity directed against bacteria involved in caries and gingivitis.
Food & Function
, 9
(6)
pp. 3489-3499.
10.1039/c7fo01727h.
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Abstract
The present study investigated the compounds present in the low molecular mass fraction of Lentinus edodes mushroom (shiitake) extract and their anti-virulence activity against oral pathogens (reference and clinical Streptococcus mutans, Actinomyces naeslundii, and Prevotella intermedia strains). Oxalic, succinic, and quinic acids, and adenine, inosine, and uridine were identified by HPLC-DAD-ESI-MS/MS. Their anti-biofilm production and preformed biofilm disaggregation activities were studied using commercial standard compounds at different concentrations. As regards S. mutans, the highest activity was shown by adenine at 5 mg mL−1 both in the biofilm inhibition (BI 50%) and biofilm disaggregation tests (BD 20%). Considering A. naeslundii, BI values close to 80% were registered for oxalic acid at 1 mg mL−1 and 2 mg mL−1 and BD 50% for quinic acid at 3 mg mL−1. A weaker activity was found against P. intermedia. Furthermore, different mixtures of the commercial standards were tested showing that the activity of a compound can be strongly and sometimes negatively affected by the presence of the other compounds.
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