Zambaiti, E;
Scottoni, F;
Rizzi, E;
Russo, S;
Deguchi, K;
Eaton, S;
Pellegata, AF;
(2019)
Whole rat stomach decellularisation using a detergent-enzymatic protocol.
Whole rat stomach decellularisation using a detergent-enzymatic protocol
, 35
(1)
pp. 21-27.
10.1007/s00383-018-4372-8.
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Abstract
BACKGROUND: Conditions leading to reduced gastric volume are difficult to manage and are associated to poor quality-of-life. Stomach augmentation using a tissue-engineered stomach is a potential solution to restore adequate physiology and food reservoir. Aim of this study was to evaluate the decellularisation of whole rat stomach using a detergent-enzymatic protocol. METHODS: Stomachs harvested from rats were decellularised through luminal and vascular cannulation using 24-h detergent-enzymatic treatment and completely characterized by appropriate staining, DNA and Extracellular matrix -component quantifications. RESULTS: The detergent-enzymatic protocol allows a complete decellularisation of the gastric tissue, with a complete removal of the DNA with two cycles as confirmed by both quantifications and histological analysis. Extracellular matrix components, collagen, fibronectin, laminin and elastin, were optimally preserved by the treatment, while glycosaminoglycans were reduced. CONCLUSION: Gastric tissue can be efficiently decellularised. Scaffolds retained original structure and important components that could enhance integration with other tissues for in vivo transplant. The use of naturally derived material could be potentially considered for the treatment of both congenital and acquired conditions.
| Type: | Article |
|---|---|
| Title: | Whole rat stomach decellularisation using a detergent-enzymatic protocol |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1007/s00383-018-4372-8 |
| Publisher version: | https://doi.org/10.1007/s00383-018-4372-8 |
| Language: | English |
| Additional information: | © The Author(s) 2018. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/). |
| Keywords: | Stomach, Decellularisation, Tissue engineering, Extracellular matrix, Scaffold, Microgastria, Gastrectomy |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > UCL GOS Institute of Child Health UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > UCL GOS Institute of Child Health > Developmental Biology and Cancer Dept |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10062666 |
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