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The Role of Mitotic Cell-Substrate Adhesion Re-modeling in Animal Cell Division

Dix, CL; Matthews, HK; Uroz, M; McLaren, S; Wolf, L; Heatley, N; Win, Z; ... Baum, B; + view all (2018) The Role of Mitotic Cell-Substrate Adhesion Re-modeling in Animal Cell Division. Developmental Cell , 45 (1) pp. 132-145. 10.1016/j.devcel.2018.03.009. Green open access

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Abstract

Animal cells undergo a dramatic series of shape changes as they divide, which depend on re-modeling of cell-substrate adhesions. Here, we show that while focal adhesion complexes are disassembled during mitotic rounding, integrins remain in place. These integrin-rich contacts connect mitotic cells to the underlying substrate throughout mitosis, guide polarized cell migration following mitotic exit, and are functionally important, since adherent cells undergo division failure when removed from the substrate. Further, the ability of cells to re-spread along pre-existing adhesive contacts is essential for division in cells compromised in their ability to construct a RhoGEF-dependent (Ect2) actomyosin ring. As a result, following Ect2 depletion, cells fail to divide on small adhesive islands but successfully divide on larger patterns, as the connection between daughter cells narrows and severs as they migrate away from one another. In this way, regulated re-modeling of cell-substrate adhesions during mitotic rounding aids division in animal cells.

Type: Article
Title: The Role of Mitotic Cell-Substrate Adhesion Re-modeling in Animal Cell Division
Open access status: An open access version is available from UCL Discovery
DOI: 10.1016/j.devcel.2018.03.009
Publisher version: https://doi.org/10.1016/j.devcel.2018.03.009
Language: English
Additional information: This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions.
Keywords: division, mitotic-rounding, integrin-based adhesion, cytokinesis
UCL classification: UCL
UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Lab for Molecular Cell Bio MRC-UCL
URI: https://discovery.ucl.ac.uk/id/eprint/10062651
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