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Getting Drugs through Small Pores: Exploiting the Porins Pathway in Pseudomonas aeruginosa

Samanta, S; Bodrenko, I; Acosta-Gutierrez, S; D'Agostino, T; Pathania, M; Ghai, I; Schleberger, C; ... Ceccarelli, M; + view all (2018) Getting Drugs through Small Pores: Exploiting the Porins Pathway in Pseudomonas aeruginosa. ACS Infectious Diseases , 4 (10) pp. 1519-1528. 10.1021/acsinfecdis.8b00149. Green open access

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Abstract

Understanding molecular properties of outer membrane channels of Gram-negative bacteria is of fundamental significance as they are the entry point of polar antibiotics into bacteria. Outer membrane proteomics revealed OccK8 (OprE) to be among the five most expressed substrate specific channels of the clinically important Pseudomonas aeruginosa. The high-resolution X-ray structure and electrophysiology highlighted a very narrow pore. However, experimental in vitro methods showed the transport of natural amino acids and antibiotics, among them ceftazidime. We used molecular dynamics simulations to reveal the importance of the physicochemical properties of ceftazidime in modulating the translocation through OccK8, proposing a structure–function relationship. As in general porins, the internal electric field favors the translocation of polar molecules by gainful energy compensation in the central constriction region. Importantly, the comparatively narrow OccK8 pore can undergo a substrate-induced expansion to accommodate relatively large-sized substrates.

Type: Article
Title: Getting Drugs through Small Pores: Exploiting the Porins Pathway in Pseudomonas aeruginosa
Open access status: An open access version is available from UCL Discovery
DOI: 10.1021/acsinfecdis.8b00149
Publisher version: http://doi.org/10.1021/acsinfecdis.8b00149
Language: English
Additional information: This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions.
Keywords: outer membrane, porins, antibiotics transport, crystal structure, molecular simulations
UCL classification: UCL
UCL > Provost and Vice Provost Offices > UCL BEAMS
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences > Dept of Chemistry
URI: https://discovery.ucl.ac.uk/id/eprint/10062599
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