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A Novel Microfluidic Point-of-Care Biosensor System on Printed Circuit Board for Cytokine Detection

Evans, D; Papadimitriou, KI; Vasilakis, N; Pantelidis, P; Kelleher, P; Morgan, H; Prodromakis, T; (2018) A Novel Microfluidic Point-of-Care Biosensor System on Printed Circuit Board for Cytokine Detection. Sensors , 18 (11) 10.3390/s18114011. Green open access

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Abstract

Point of Care (PoC) diagnostics have been the subject of considerable research over the last few decades driven by the pressure to detect diseases quickly and effectively and reduce healthcare costs. Herein, we demonstrate a novel, fully integrated, microfluidic amperometric enzyme-linked immunosorbent assay (ELISA) prototype using a commercial interferon gamma release assay (IGRA) as a model antibody binding system. Microfluidic assay chemistry was engineered to take place on Au-plated electrodes within an assay cell on a printed circuit board (PCB)-based biosensor system. The assay cell is linked to an electrochemical reporter cell comprising microfluidic architecture, Au working and counter electrodes and a Ag/AgCl reference electrode, all manufactured exclusively via standard commercial PCB fabrication processes. Assay chemistry has been optimised for microfluidic diffusion kinetics to function under continual flow. We characterised the electrode integrity of the developed platforms with reference to biological sampling and buffer composition and subsequently we demonstrated concentration-dependent measurements of H₂O₂ depletion as resolved by existing FDA-validated ELISA kits. Finally, we validated the assay technology in both buffer and serum and demonstrate limits of detection comparable to high-end commercial systems with the addition of full microfluidic assay architecture capable of returning diagnostic analyses in approximately eight minutes.

Type: Article
Title: A Novel Microfluidic Point-of-Care Biosensor System on Printed Circuit Board for Cytokine Detection
Location: Switzerland
Open access status: An open access version is available from UCL Discovery
DOI: 10.3390/s18114011
Publisher version: http://doi.org/10.3390/s18114011
Language: English
Additional information: Copyright © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
Keywords: PCB biosensors, cytokine detection, eELISA, lab-on-PCB, microfluidics, point-of-care diagnostics
UCL classification: UCL
UCL > Provost and Vice Provost Offices > UCL BEAMS
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science
URI: https://discovery.ucl.ac.uk/id/eprint/10062569
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