Ahmed, AA;
Luo, CJ;
Perez-Garrido, S;
Browse, CR;
Thrasivoulou, C;
Stoyanov, SD;
Smoukov, SK;
(2019)
3D cancer cell culture in high‐yield multi‐scale scaffolds by shear spinning.
Biotechnology Progress
, 35
(2)
, Article e2750. 10.1002/btpr.2750.
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Abstract
Polymeric scaffolds comprising two size scales of microfibres and submicron fibres can better support 3D cell growth in tissue engineering, making them an important class of healthcare material. However, a major manufacturing barrier hampers their translation into wider practical use: scalability. Traditional production of two‐scale scaffolds by electrospinning is slow and costly. For day‐to‐day cell cultures, the scaffolds need to be affordable, made in high yield to drive down cost. Combining expertise from academia and industry from the UK and USA, this study employs a new series of high‐yield, low‐cost scaffolds made by shear‐spinning for tissue engineering. The scaffolds comprise interwoven sub‐micron fibres and microfibres throughout as observed under scanning electron microscopy and demonstrate good capability to support cell culturing for tumour modelling. Three model human cancer cell lines (HEK293, A549 and MCF‐7) with stable expression of GFP were cultured in the scaffolds and found to exhibit efficient cell attachment and sustained 3D growth and proliferation for 30 days. Cryosection and multiphoton fluorescence microscopy confirmed the formation of compact 3D cell clusters throughout the scaffolds. In addition, comparative growth curves of 2D and 3D cell cultures show significant cell‐type‐dependent differences. This work applies high‐yield shear‐spun scaffolds in mammalian tissue engineering and brings practical, affordable applications of multi‐scale scaffolds closer to reality.
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