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A Toolkit to Quantify Target Compounds in Thin-Layer-Chromatography Experiments

Mac Fhionnlaoich, N; Ibsen, S; Serrano, LA; Taylor, A; Qi, R; Guldin, S; (2018) A Toolkit to Quantify Target Compounds in Thin-Layer-Chromatography Experiments. Journal of Chemical Education , 95 (12) pp. 2191-2196. 10.1021/acs.jchemed.8b00144. Green open access

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Abstract

Thin-layer chromatography (TLC) is one of the basic analytical procedures in chemistry and allows the demonstration of various chemical principles in an educational setting. An often-overlooked aspect of TLC is the capability to quantify isolated target compounds in an unknown sample. Here, we present a suitable route to implement quantitative analysis in a lesson plan. We provide both a stand-alone software and an online webapp that allow students to obtain quantitative information from a developed TLC plate and present two suitable experiments, namely, the absorbance-based quantification of the colorant Sudan IV and the fluorescence-based quantification of rhodamine 6G, a fluorophore widely used in biotechnology. Students conduct TLC experiments following established protocols, take pictures of their TLC plates with mobile phones, and subsequently quantify the different compounds in the separate bands they observe.

Type: Article
Title: A Toolkit to Quantify Target Compounds in Thin-Layer-Chromatography Experiments
Open access status: An open access version is available from UCL Discovery
DOI: 10.1021/acs.jchemed.8b00144
Publisher version: https://doi.org/10.1021/acs.jchemed.8b00144
Language: English
Additional information: Copyright © Creative Commons Attribution (CC-BY) License, which permits unrestricted use, distribution and reproduction in any medium, provided the author and source are cited.
Keywords: Analytical Chemistry; Computer-Based Learning; Continuing Education; Demonstrations; Environmental Chemistry; First-Year Undergraduate/General; General Public; Graduate Education/Research; Hands-On Learning/Manipulatives; High School/Introductory Chemistry; Inquiry-Based/Discovery Learning; Laboratory Computing/Interfacing; Laboratory Equipment/Apparatus; Laboratory Instruction; Microscale Lab; Problem Solving/Decision Making; Public Understanding/Outreach; Quantitative Analysis; Second-Year Undergraduate; Student-Centered Learning; Thin Layer Chromatography; Upper-Division Undergraduate
UCL classification: UCL
UCL > Provost and Vice Provost Offices > UCL BEAMS
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Chemical Engineering
URI: https://discovery.ucl.ac.uk/id/eprint/10061140
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