Llombart, V;
Alejandro Trejo, S;
Bronsoms, S;
Morancho, A;
Ma, F;
Faura, J;
Garcia-Berrocoso, T;
... Montaner, J; + view all
(2017)
Profiling and identification of new proteins involved in brain ischemia using MALDI-imaging-mass-spectrometry.
Journal of Proteomics
, 152
pp. 243-253.
10.1016/j.jprot.2016.11.014.
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Abstract
The identification of proteins involved in brain ischemia might allow the discovery of putative biomarkers or therapeutic targets for ischemic stroke. Our aim is to study the distribution of proteins within mouse brain after an ischemic insult using MALDI imaging-mass-spectrometry and to identify relevant proteins involved in brain damage. We occluded the middle cerebral artery of C57BL/6J mice. Brain slices were analyzed by MALDI-TOF and infarct (IC) and contralateral (CL) regions were compared using ClinProTools. The ion distribution maps of relevant m/z values were obtained by FlexImagin3.0. Protein identification was conducted through a bottom-up approach consisting on complementary sample fractionation methods. Some identifications were confirmed by immunohistochemistry and western blot. We identified 102 m/z values with different abundances between IC and CL (p < 0.05), from which 21 m/z peaks were selected as more relevant. Thirteen of them were found increased in the infarct region and 4 m/z values showed AUC > 90% between IC and CL. Identification analyses confirmed altered expressions of ATP5i, COX6C and UMP-CMP kinase in IC compared to CL. Biological significance: Using MALDI-IMS we identified for the first time new proteins that might be involved in brain ischemia representing potential diagnostic biomarkers or target molecules for neurological recovery.
| Type: | Article |
|---|---|
| Title: | Profiling and identification of new proteins involved in brain ischemia using MALDI-imaging-mass-spectrometry |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1016/j.jprot.2016.11.014 |
| Publisher version: | http://doi.org/10.1016/j.jprot.2016.11.014 |
| Language: | English |
| Additional information: | This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions. |
| Keywords: | MALDI-imaging, Cerebral ischemiat, MCAOIschemic stroke, Biomarkers |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Cancer Institute UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Cancer Institute > Research Department of Haematology |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10060565 |
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