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Three-dimensional cell culture: from evolution to revolution

Alhaque, S; Themis, M; Rashidi, H; (2018) Three-dimensional cell culture: from evolution to revolution. Philosophical Transactions B: Biological Sciences , 373 (1750) , Article 20170216. 10.1098/rstb.2017.0216. Green open access

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Abstract

Recent advances in the isolation of tissue-resident adult stem cells and the identification of inductive factors that efficiently direct differentiation of human pluripotent stem cells along specific lineages have facilitated the development of high-fidelity modelling of several tissues in vitro. Many of the novel approaches have employed self-organizing three-dimensional (3D) culturing of organoids, which offer several advantages over conventional two-dimensional platforms. Organoid technologies hold great promise for modelling diseases and predicting the outcome of drug responses in vitro. Here, we outline the historical background and some of the recent advances in the field of three-dimensional organoids. We also highlight some of the current limitations of these systems and discuss potential avenues to further benefit biological research using three-dimensional modelling technologies. This article is part of the theme issue ‘Designer human tissue: coming to a lab near you.

Type: Article
Title: Three-dimensional cell culture: from evolution to revolution
Open access status: An open access version is available from UCL Discovery
DOI: 10.1098/rstb.2017.0216
Publisher version: https://doi.org/10.1098/rstb.2017.0216
Language: English
Additional information: This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions.
Keywords: Science & Technology, Life Sciences & Biomedicine, Biology, Life Sciences & Biomedicine - Other Topics, pluripotent stem cells, organoids, three-dimensional culture, PLURIPOTENT STEM-CELLS, IN-VITRO EXPANSION, ON-A-CHIP, INTESTINAL ORGANOIDS, CEREBRAL ORGANOIDS, CONE PHOTORECEPTORS, SPHEROID FORMATION, BASEMENT-MEMBRANE, EPITHELIAL-CELLS, PROGENITOR CELLS
UCL classification: UCL
UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > UCL GOS Institute of Child Health
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > UCL GOS Institute of Child Health > Developmental Biology and Cancer Dept
URI: https://discovery.ucl.ac.uk/id/eprint/10060411
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