Figini, M;
Wang, X;
Lyu, T;
Su, Z;
Wang, B;
Sun, C;
Shangguan, J;
... Zhang, Z; + view all
(2018)
Diffusion Mri Biomarkers Predict the Outcome O Irreversible Electroporation in a Pancreatic Tumor Mouse Model.
American Journal of Cancer Research
, 8
(8)
pp. 1615-1623.
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Abstract
The purpose of this work is to explore the potential contribution of diffusion MRI to predict the effects of irreversible electroporation (IRE) in a pancreatic ductal adenocarcinoma (PDAC) mouse model. Thirteen mice were injected with Panc-02 PDAC cells in both flanks. One tumor was treated with IRE when it reached a diameter of about 5 mm. T2- and diffusion-weighted MRI sequences were acquired before IRE treatment and 1, 3 and 7 days later. The mice were euthanized 1 day (n = 6) or 2 weeks (n = 7) after treatment. The tumors were excised and stained with H&E, caspase-3, CD-3, F4/80. The volume and the mean and standard deviation of the apparent diffusion coefficient (ADC) were compared between treated and untreated lesions and correlated with histology-derived measures. At 1-day post-treatment, a dramatic ADC increase (+50.81%, P <0.05) was found in ablated lesions, strongly correlated with apoptosis (τ = 0.90). At later time points the ADC returned to pre-treatment values, though histopathology showed a quite different scenario compared to the untreated controls. The ADC standard deviation measured within the treated tumors 1 day after IRE treatment had a strong negative correlation with the number of tumor cells found 14 days later (τ = 0.80). There was also a strong correlation between 1-day ADC and 14-day apoptosis in untreated tumors (τ = 0.95). In conclusion, diffusion MRI is sensitive to the short-term effects of IRE in PDAC tumors, and can help predict the long-term treatment outcome.
| Type: | Article |
|---|---|
| Title: | Diffusion Mri Biomarkers Predict the Outcome O Irreversible Electroporation in a Pancreatic Tumor Mouse Model |
| Open access status: | An open access version is available from UCL Discovery |
| Publisher version: | http://www.ajcr.us/AJCR_V8N8.html |
| Language: | English |
| Additional information: | This version is the version of record. For information on re-use, please refer to the publisher’s terms and conditions. |
| Keywords: | Irreversible electroporation, diffusion MRI, apparent diffusion coefficient, pancreatic cancer, treatment outcome, apoptosis, early prediction, mouse model. |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > UCL BEAMS UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Computer Science |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10059892 |
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