UCL Discovery
UCL home » Library Services » Electronic resources » UCL Discovery

Generation of a Cancer Testis Antigen Mcherry Reporter Hct116 Colorectal Carcinoma Cell Line

Chhetri, JB; Drousioti, E; Guerra-Assunção, JA; Herrero, J; Ooi, SKT; (2018) Generation of a Cancer Testis Antigen Mcherry Reporter Hct116 Colorectal Carcinoma Cell Line. Heliyon , 4 (10) , Article e00858. 10.1016/j.heliyon.2018.e00858. Green open access

[thumbnail of 1-s2.0-S2405844018319996-main.pdf]
Preview
Text
1-s2.0-S2405844018319996-main.pdf - Published Version

Download (1MB) | Preview

Abstract

In the context of cancer immunotherapy, agents that target the immune system to cancer cells need to fulfil two criteria: 1) that they are only expressed on the desired target cell and 2) that they can elicit a potent immunological response. Cancer Testis Antigens are a large disparate family of factors ordinarily expressed in the germ-line but aberrantly expressed across multiple types of cancer. The ability to enforce their expression on tumour cells is an attractive strategy that could render such cells potent targets of the immune system, but very little is known about their regulation. We describe the generation of an mCherry reporter cell line using HCT116 colorectal carcinoma cells that we anticipate will be useful for screen-based approaches to identify novel regulators of CTA expression. Discoveries arising from their use could in future be exploited to enhance tumour cell immunogenicity and improve cancer immuno-therapy.

Type: Article
Title: Generation of a Cancer Testis Antigen Mcherry Reporter Hct116 Colorectal Carcinoma Cell Line
Open access status: An open access version is available from UCL Discovery
DOI: 10.1016/j.heliyon.2018.e00858
Publisher version: https://doi.org/10.1016/j.heliyon.2018.e00858
Language: English
Additional information: This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
Keywords: Cell biology, Developmental biology
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Cancer Institute
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Cancer Institute > Research Department of Cancer Bio
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Infection and Immunity
URI: https://discovery.ucl.ac.uk/id/eprint/10059803
Downloads since deposit
85Downloads
Download activity - last month
Download activity - last 12 months
Downloads by country - last 12 months

Archive Staff Only

View Item View Item