Man, WH;
van Dongen, TMA;
Venekamp, RP;
Pluimakers, VG;
Chu, MLJN;
van Houten, MA;
Sanders, EAM;
... Bogaert, D; + view all
(2018)
Respiratory Microbiota Predicts Clinical Disease Course of Acute Otorrhea in Children With Tympanostomy Tubes.
The Pediatric Infectious Disease Journal
10.1097/INF.0000000000002215.
(In press).
Preview |
Text
Schilder_PIDJ_LOT_Merged_Revision.pdf - Accepted Version Download (3MB) | Preview |
Abstract
BACKGROUND: Acute otitis media (AOM) is one of the most common childhood infections, generally thought to be caused by ascension of bacteria from the nasopharynx (NP) to the middle ear. Using 16S rRNA-based sequencing, we evaluated the relationship between the NP and middle ear fluid (MEF) microbiota in children with acute otitis media with tympanostomy tubes (AOMT) as a proxy for AOM, and explored whether microbiota profiling predicts natural disease course. METHODS: Microbiota profiles of paired NP and MEF samples of 94 children aged below five years with uncomplicated AOMT were determined. RESULTS: Local diversity (p<0·001) and overall microbiota composition (p<0·001) of NP and MEF samples differed significantly, though paired NP and MEF samples were much more similar than unpaired samples (p<0·001). High qualitative agreement between the presence of individual bacteria in both niches was observed. Abundances of Pseudomonas aeruginosa, Staphylococcus aureus, Streptococcus pyogenes, Turicella otitidis, Klebsiella pneumoniae, and Haemophilus spp. were strongly correlated between the two niches. Additionally, P. aeruginosa, S. aureus, T. otitidis and Streptococcus pneumoniae abundance in NP were predictive of the presence of a range of oral types of bacteria in MEF. Interestingly, there was no association between Moraxella catarrhalis in NP and MEF samples, which was highly present in NP but virtually absent in MEF. Finally, the NP microbiota composition could predict duration of AOMT, even better than MEF microbiota. CONCLUSIONS: We observed substantial correlations between paired NP and MEF microbiota in children with AOMT. Our data also suggest that NP microbiota profiling deserves further exploration as tool for future treatment decisions.
| Type: | Article |
|---|---|
| Title: | Respiratory Microbiota Predicts Clinical Disease Course of Acute Otorrhea in Children With Tympanostomy Tubes |
| Location: | United States |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1097/INF.0000000000002215 |
| Publisher version: | http://dx.doi.org/10.1097/INF.0000000000002215 |
| Language: | English |
| Additional information: | This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions. |
| Keywords: | respiratory microbiota; nasopharynx; middle ear fluid; acute otitis media; childhood infections |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > The Ear Institute |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10059486 |
Archive Staff Only
 |
View Item |
