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Use of LC‐MS analysis to elucidate by‐products of niacinamide transformation following in vitro skin permeation studies

Sil, BC; Moore, DJ; Lane, ME; (2018) Use of LC‐MS analysis to elucidate by‐products of niacinamide transformation following in vitro skin permeation studies. International Journal of Cosmetic Science , 40 (5) pp. 525-529. 10.1111/ics.12486. Green open access

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Abstract

OBJECTIVE: To explore and elucidate the formation of niacinamide (NIA) by-products during in vitro skin permeation studies using liquid chromatography coupled to mass spectrometry (LC-MS) analysis. METHODS: Porcine skin permeation studies of various NIA formulations were conducted using Franz diffusion cells for a period of 24 hours. NIA by-products were identified by LC, extracted and further qualitatively analysed by LC-MS. RESULTS: Analysis and characterisation of NIA by-products using LC-MS resulted in the identification of different molecular entities with similar structures to NIA. The most prevalent molecular specie in this study was 1,4,5,6-tetrahydropyridine-3-carboxamide with the highest ion abundance. Other structural NIA analogues were also identified and reported, namely piperidine-3-carboxamide and 1,4-dihydropyridine-3-carboxamide. None of these NIA derivatives were detected in stability studies of NIA in the medium used as the receptor phase, phosphate buffered saline (PBS), that had not been in contact with skin. CONCLUSION: The comparatively low recovery of NIA following in vitro mass-balance and permeation studies for pseudo-finite and finite dosing of the active compared with infinite dosing is attributed to chemical derivatisation of the molecule during skin penetration. These findings reported here will allow the development of more sensitive methods to ensure full mass balance recovery of NIA following topical application of NIA preparations.

Type: Article
Title: Use of LC‐MS analysis to elucidate by‐products of niacinamide transformation following in vitro skin permeation studies
Location: England
Open access status: An open access version is available from UCL Discovery
DOI: 10.1111/ics.12486
Publisher version: https://doi.org/10.1111/ics.12486
Language: English
Additional information: This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions.
Keywords: Chemical analysis, Liquid chromatography mass-spectroscopy (LC-MS), NIA by-products, Niacinamide
UCL classification: UCL
UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > UCL School of Pharmacy
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > UCL School of Pharmacy > Pharmaceutics
URI: https://discovery.ucl.ac.uk/id/eprint/10058538
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