UCL Discovery
UCL home » Library Services » Electronic resources » UCL Discovery

Intercellular Ca²⁺ signalling in the adult mouse cochlea

Sirko, P; Gale, JE; Ashmore, JF; (2019) Intercellular Ca²⁺ signalling in the adult mouse cochlea. The Journal of Physiology , 597 (1) pp. 303-317. 10.1113/JP276400. Green open access

[thumbnail of Ashmore_Intercellular Ca2+ signalling in the adult mouse cochlea_VoR.pdf]
Preview
Text
Ashmore_Intercellular Ca2+ signalling in the adult mouse cochlea_VoR.pdf

Download (1MB) | Preview

Abstract

We have investigated wave‐like cytoplasmic calcium (Ca²⁺) signalling in an ex vivo preparation of the adult mouse organ of Corti. Two types of intercellular Ca²⁺ waves that differ in propagation distance and speed were observed. One type was observed to travel up to 100 μm with an average velocity of 7 μm/s. Such waves were initiated by local tissue damage in the outer hair cell region. The propagation distance was decreased when the purinergic receptor antagonists pyridoxalphosphate‐6‐azophenyl‐2′,4′‐disulfonic acid (PPADS; 50 μm) or suramin (150 μm) were added to the extracellular buffer. Immunocytochemical analysis and experiments with calcium indicator dyes showed that both P2X and P2Y receptors were present in supporting cells. A second class of waves identified to travel longitudinally along the organ of Corti propagated at a lower velocity of 1–3 μm/s. These ‘slow’ Ca²⁺ waves were particularly evident in the inner sulcus and Deiters’ cells. They travelled for distances of up to 500 μm. The slow Ca²⁺ signalling varied periodically (approximately one wave every 10 min) and was maintained for more than 3 h. The slow waves were not affected by apyrase, or by the P2 receptor agonists suramin (150 μm) or PPADS (50 μm) but were blocked by the connexin channel blockers octanol (1 mm) and carbenoxolone (100 μm). It is proposed that the observed Ca²⁺ waves might be a physiological response to a change in extracellular environment and may be involved in critical gene regulation activities in the supporting cells of the cochlea.

Type: Article
Title: Intercellular Ca²⁺ signalling in the adult mouse cochlea
Open access status: An open access version is available from UCL Discovery
DOI: 10.1113/JP276400
Publisher version: https://doi.org/10.1113/JP276400
Language: English
Additional information: © 2018 The Authors. The Journal of Physiology published by John Wiley & Sons Ltd on behalf of The Physiological Society. This is an open access article under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/).
Keywords: Cochlea, hearing, calcium waves, supporting cells
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > The Ear Institute
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Neuro, Physiology and Pharmacology
URI: https://discovery.ucl.ac.uk/id/eprint/10058483
Downloads since deposit
127Downloads
Download activity - last month
Download activity - last 12 months
Downloads by country - last 12 months

Archive Staff Only

View Item View Item