UCL Discovery
UCL home » Library Services » Electronic resources » UCL Discovery

Effects of bovine serum albumin on light activated antimicrobial surfaces

Oliveira Lourenco, CR; Macdonald, T; Gavriilidis, A; Allan, E; Macrobert, A; Parkin, I; (2018) Effects of bovine serum albumin on light activated antimicrobial surfaces. Rsc Advances , 8 pp. 34252-34258. 10.1039/c8ra04361b. Green open access

[thumbnail of Effects of Bovine Serum Albumin on Light Activated Antimicrobial Surfaces-Published paper.pdf]
Preview
Text
Effects of Bovine Serum Albumin on Light Activated Antimicrobial Surfaces-Published paper.pdf - Published version

Download (920kB) | Preview

Abstract

Bovine serum albumin (BSA) is currently recommended as an interfering substance to emulate organic soiling, in evaluating the efficacy of disinfectants. The European Standard recommends 0.03% BSA to test clean conditions and 0.3% for dirty conditions. Reactive oxygen species are known to exert excellent antimicrobial activity with low specificity against a broad range of pathogens. Herein, we present our data from the first study of the effects of the addition of BSA on the antibacterial activity of light activated antimicrobial surfaces. Light activated antimicrobial surfaces were made from polyurethane swell-encapsulated with gold nanoparticles (AuNPs) coated with the light active triarylmethane dye, crystal violet (PU-AuNP-CV). The antibacterial efficacy of the antimicrobial substrates was tested against two strains of Staphylococcus aureus 8325-4, a well-characterised laboratory strain and MRSA 4742, a recent clinical isolate, in the presence of 0.1% to 1% BSA by irradiating the substrates with a fluorescent lamp (300 lux). After 6 hours of irradiation, the number of surviving bacteria was determined. The results showed that BSA reduced the antibacterial efficacy of all the PU-AuNP-CV surfaces with increasing BSA concentrations resulting in a progressive reduction in antibacterial activity towards the bacteria tested. However, the light activated surfaces did perform well at 0.1 and 0.25% BSA levels, showing they may have potential for real world environments with low levels of organic soiling.

Type: Article
Title: Effects of bovine serum albumin on light activated antimicrobial surfaces
Open access status: An open access version is available from UCL Discovery
DOI: 10.1039/c8ra04361b
Publisher version: http://dx.doi.org/10.1039/c8ra04361b
Language: English
Additional information: This article is publisher under a Creative Commons CC BY (https://creativecommons.org/licenses/by/3.0/) licence
UCL classification: UCL
UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Surgery and Interventional Sci
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Surgery and Interventional Sci > Department of Surgical Biotechnology
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Eastman Dental Institute
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Eastman Dental Institute > Microbial Diseases
UCL > Provost and Vice Provost Offices > UCL BEAMS
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Chemical Engineering
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences > Dept of Chemistry
URI: https://discovery.ucl.ac.uk/id/eprint/10057819
Downloads since deposit
66Downloads
Download activity - last month
Download activity - last 12 months
Downloads by country - last 12 months

Archive Staff Only

View Item View Item