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Immune receptors CD40 and CD86 in oral keratinocytes and implications for oral lichen planus

Marshall, A; Celentano, A; Cirillo, N; Mirams, M; McCullough, M; Porter, S; (2017) Immune receptors CD40 and CD86 in oral keratinocytes and implications for oral lichen planus. Journal of Oral Science , 59 (3) pp. 373-382. 10.2334/josnusd.16-0334. Green open access

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Abstract

Lichen planus (LP) is a chronic T-cell-mediated mucocutaneous inflammatory disease that targets stratified epithelia, including those lining the oral cavity. The intraoral variant of LP (OLP) is associated with interferon (IFN)-γ production by infiltrating T lymphocytes; however, the role of epithelial cells in the etiopathogenesis OLP is not completely understood. There is however a growing body of evidence regarding the involvement of epithelial-derived cytokines, immune receptors, and costimulatory molecules in the pathobiological processes that promote and sustain OLP. In the present study, we used a reverse transcriptase-polymerase chain reaction assay to assess whether CD40—a receptor found mainly on antigen presenting cells—and the costimulatory molecule CD86 were expressed in oral keratinocytes (three strains of primary normal oral keratinocytes and the H357 cell line) in the presence or absence of IFN-γ. To further characterize the involvement of CD40 in OLP, expression and distribution of receptor and ligand (CD40/CD154) in tissues from OLP were evaluated by immunohistochemistry. The present results are the first to show that both CD40 and CD86 are constitutively expressed at low levels in oral keratinocytes and that their expression was enhanced by IFN-γ stimulation. The intensity of CD40 staining in OLP tissues was strong. Taken together, the results strongly suggest that CD40 and CD86 play a role in the pathophysiology of oral inflammatory diseases such as OLP.

Type: Article
Title: Immune receptors CD40 and CD86 in oral keratinocytes and implications for oral lichen planus
Open access status: An open access version is available from UCL Discovery
DOI: 10.2334/josnusd.16-0334
Publisher version: http://dx.doi.org/10.2334/josnusd.16-0334
Language: English
Additional information: This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions.
Keywords: Science & Technology, Life Sciences & Biomedicine, Technology, Dentistry, Oral Surgery & Medicine, Materials Science, Biomaterials, Materials Science, CD40, CD86, oral lichen planus, oral inflammation, regulatory T-cells, mucosa, SQUAMOUS-CELL CARCINOMA, EXPERIMENTAL AUTOIMMUNE ENCEPHALOMYELITIS, INTESTINAL EPITHELIAL-CELLS, HEAT-SHOCK PROTEINS, IN-SITU EXPRESSION, CYTOTOXIC T-CELLS, FUNCTIONAL EXPRESSION, LANGERHANS CELLS, INTERFERON-GAMMA, DENDRITIC CELLS
UCL classification: UCL
UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Eastman Dental Institute
URI: https://discovery.ucl.ac.uk/id/eprint/10057769
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