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The use of a surface active agent in the protection of a fusion protein during bioprocessing

Blas, P; Tolner, B; Ward, J; Chester, K; Hoare, M; (2018) The use of a surface active agent in the protection of a fusion protein during bioprocessing. Biotechnology and Bioengineering , 115 (11) pp. 2760-2770. 10.1002/bit.26817. Green open access

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Abstract

The bioprocessing of a fusion protein is characterised by low yields and at a series of recovery and purification stages that leads to an overall 90% loss. Much of this apparent loss is due to the denaturation of a protein, missing a vital affinity ligand. However, there is evidence of the protection of degradation products which occurs in the presence of shear plus air/liquid interfaces. This study seeks out to characterise the loss and use ultra‐scale‐down studies to predict its occurrence and hence shows these may be diminished by the use of protective reagents such as Pluronic F68.

Type: Article
Title: The use of a surface active agent in the protection of a fusion protein during bioprocessing
Location: United States
Open access status: An open access version is available from UCL Discovery
DOI: 10.1002/bit.26817
Publisher version: https://doi.org/10.1002/bit.26817
Language: English
Additional information: © 2018 The Authors. Biotechnology and Bioengineering Published by Wiley Periodicals, Inc. This is an open access article under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/).
Keywords: degradation, fusion protein, pluronic acid, protection, surface active agent, USD shear device
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Cancer Institute
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Cancer Institute > Research Department of Oncology
UCL > Provost and Vice Provost Offices > UCL BEAMS
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Biochemical Engineering
URI: https://discovery.ucl.ac.uk/id/eprint/10057739
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