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In Vitro Fertilization of Mouse Oocyte

Chaigne, A; (2018) In Vitro Fertilization of Mouse Oocyte. Methods in Molecular Biology , 1818 pp. 37-42. 10.1007/978-1-4939-8603-3_5. Green open access

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Abstract

The early development of embryos is a widely studied process. Fertilization is the consequence of the fusion of two haploid gametes, the oocyte and the sperm. In mammals, such as human and mouse, this occurs in the female genital tracks. However, imaging in utero is still very limited, which prompts the use of extra utero techniques. In particular, in vitro fertilization provides a quick and simple way to study the very early steps of mouse embryo development. Here, I describe a simple and hands-on protocol to perform in vitro fertilization using the mouse as a model system.

Type: Article
Title: In Vitro Fertilization of Mouse Oocyte
Location: United States
Open access status: An open access version is available from UCL Discovery
DOI: 10.1007/978-1-4939-8603-3_5
Publisher version: http://doi.org/10.1007/978-1-4939-8603-3_5
Language: English
Additional information: This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions.
Keywords: In vitro fertilization, Mouse oocyte, Superovulation, Zygote
UCL classification: UCL
UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Lab for Molecular Cell Bio MRC-UCL
URI: https://discovery.ucl.ac.uk/id/eprint/10057419
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