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Inositol Phosphates Purification Using Titanium Dioxide Beads

Wilson, MS; Saiardi, A; (2018) Inositol Phosphates Purification Using Titanium Dioxide Beads. Bio-Protocol , 8 (15) , Article 2959. 10.21769/BioProtoc.2959. Green open access

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Abstract

Inositol phosphates (IPs) comprise a family of ubiquitous eukaryotic signaling molecules. They have been linked to the regulation of a pleiotropy of important cellular activities, but low abundance and detection difficulties have hampered our understanding. Here we present a method to purify and enrich IPs or other phosphate-rich metabolites from mammalian cells or other sample types. Acid-extracted IPs from cells bind selectively via their phosphate groups to titanium dioxide beads. After washing, the IPs are easily eluted from the beads by increasing the pH. This technique, in combination with downstream analytical methods such as PAGE or SAX-HPLC, opens unprecedented investigative possibilities, allowing appropriate analysis of IPs from virtually any biological or non-biological source.

Type: Article
Title: Inositol Phosphates Purification Using Titanium Dioxide Beads
Location: United States
Open access status: An open access version is available from UCL Discovery
DOI: 10.21769/BioProtoc.2959
Publisher version: https://doi.org/10.21769/BioProtoc.2959
Language: English
Additional information: This version is the version of record. For information on re-use, please refer to the publisher’s terms and conditions.
Keywords: Inositol polyphosphate, Inositol pyrophosphate, IP₆, IP₇, Metabolism, Metabolites, Signaling
UCL classification: UCL
UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Lab for Molecular Cell Bio MRC-UCL
URI: https://discovery.ucl.ac.uk/id/eprint/10057343
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