Wilson, MS;
Saiardi, A;
(2018)
Inositol Phosphates Purification Using Titanium Dioxide Beads.
Bio-Protocol
, 8
(15)
, Article 2959. 10.21769/BioProtoc.2959.
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Abstract
Inositol phosphates (IPs) comprise a family of ubiquitous eukaryotic signaling molecules. They have been linked to the regulation of a pleiotropy of important cellular activities, but low abundance and detection difficulties have hampered our understanding. Here we present a method to purify and enrich IPs or other phosphate-rich metabolites from mammalian cells or other sample types. Acid-extracted IPs from cells bind selectively via their phosphate groups to titanium dioxide beads. After washing, the IPs are easily eluted from the beads by increasing the pH. This technique, in combination with downstream analytical methods such as PAGE or SAX-HPLC, opens unprecedented investigative possibilities, allowing appropriate analysis of IPs from virtually any biological or non-biological source.
| Type: | Article |
|---|---|
| Title: | Inositol Phosphates Purification Using Titanium Dioxide Beads |
| Location: | United States |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.21769/BioProtoc.2959 |
| Publisher version: | https://doi.org/10.21769/BioProtoc.2959 |
| Language: | English |
| Additional information: | This version is the version of record. For information on re-use, please refer to the publisher’s terms and conditions. |
| Keywords: | Inositol polyphosphate, Inositol pyrophosphate, IP₆, IP₇, Metabolism, Metabolites, Signaling |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Lab for Molecular Cell Bio MRC-UCL |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10057343 |
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