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Left/right asymmetric collective migration of parapineal cells is mediated by focal FGF signalling activity in leading cells

Roussigné, M; Wei, L; Tsingos, E; Kuchling, F; Alkobtawi, M; Tsalavouta, M; Wittbrodt, J; ... Wilson, SW; + view all (2018) Left/right asymmetric collective migration of parapineal cells is mediated by focal FGF signalling activity in leading cells. Proceedings of the National Academy of Sciences of the United States of America , 115 (42) E9812-E9821. 10.1073/pnas.1812016115. Green open access

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Abstract

The ability of cells to collectively interpret surrounding environmental signals underpins their capacity to coordinate their migration in various contexts, including embryonic development and cancer metastasis. One tractable model for studying collective migration is the parapineal, a left-sided group of neurons that arises from bilaterally positioned precursors that undergo a collective migration to the left side of the brain. In zebrafish, the migration of these cells requires Fgf8 and, in this study, we resolve how FGF signaling correlates with—and impacts the migratory dynamics of—the parapineal cell collective. The temporal and spatial dynamics of an FGF reporter transgene reveal that FGF signaling is activated in only few parapineal cells usually located at the leading edge of the parapineal during its migration. Overexpressing a constitutively active Fgf receptor compromises parapineal migration in wild-type embryos, while it partially restores both parapineal migration and mosaic expression of the FGF reporter transgene in fgf8−/− mutant embryos. Focal activation of FGF signaling in few parapineal cells is sufficient to promote the migration of the whole parapineal collective. Finally, we show that asymmetric Nodal signaling contributes to the restriction and leftwards bias of FGF pathway activation. Our data indicate that the first overt morphological asymmetry in the zebrafish brain is promoted by FGF pathway activation in cells that lead the collective migration of the parapineal to the left. This study shows that cell-state differences in FGF signaling in front versus rear cells is required to promote migration in a model of FGF-dependent collective migration.

Type: Article
Title: Left/right asymmetric collective migration of parapineal cells is mediated by focal FGF signalling activity in leading cells
Open access status: An open access version is available from UCL Discovery
DOI: 10.1073/pnas.1812016115
Publisher version: https://doi.org/10.1073/pnas.1812016115
Language: English
Additional information: This open access article is distributed under Creative Commons Attribution-Non Commercial No Derivatives License 4.0 (CC BY-NC-ND).
Keywords: Collective cell migration, FGF signaling pathway, left/right brain asymmetry, Nodal signaling pathway, zebrafish brain development
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Cell and Developmental Biology
URI: https://discovery.ucl.ac.uk/id/eprint/10057087
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