UCL Discovery
UCL home » Library Services » Electronic resources » UCL Discovery

Therapeutic enhancement of a cytotoxic agent using Photochemical internalisation in 3D compressed collagen constructs of ovarian cancer

Mohammad-Hadi, L; Yaghini, E; Stamati, K; Loizidou, M; Macrobert, A; (2018) Therapeutic enhancement of a cytotoxic agent using Photochemical internalisation in 3D compressed collagen constructs of ovarian cancer. Acta Biomaterialia , 81 pp. 80-92. 10.1016/j.actbio.2018.09.041. Green open access

[thumbnail of Article]
Preview
Text (Article)
Mohammad Hadi_Research paper-accepted.pdf - Accepted version

Download (1MB) | Preview
[thumbnail of Supplementary Information]
Preview
Text (Supplementary Information)
Mohammad Hadi_Supplementary information-accepted.pdf - Accepted version

Download (905kB) | Preview

Abstract

Photochemical internalisation (PCI) is a method for enhancing delivery of drugs to their intracellular target sites of action. In this study we investigated the efficacy of PCI using a porphyrin photosensitiser and a cytotoxic agent on spheroid and non-spheroid compressed collagen 3D constructs of ovarian cancer versus conventional 2D culture. The therapeutic responses of two human carcinoma cell lines (SKOV3 and HEY) were compared using a range of assays including optical imaging. The treatment was shown to be effective in non-spheroid constructs of both cell lines causing a significant and synergistic reduction in cell viability measured at 48 or 96 hours post-illumination. In the larger spheroid constructs, PCI was still effective but required higher saporin and photosensitiser doses. Moreover, in contrast to the 2D and non-spheroid experiments, where comparable efficacy was found for the two cell lines, HEY spheroid constructs were found to be more susceptible to PCI and a lower dose of saporin could be used. PCI treatment was observed to induce death principally by apoptosis in the 3D constructs compared to the mostly necrotic cell death caused by PDT. At low oxygen levels (1%) both PDT and PCI were significantly less effective in the constructs.

Type: Article
Title: Therapeutic enhancement of a cytotoxic agent using Photochemical internalisation in 3D compressed collagen constructs of ovarian cancer
Open access status: An open access version is available from UCL Discovery
DOI: 10.1016/j.actbio.2018.09.041
Publisher version: https://doi.org/10.1016/j.actbio.2018.09.041
Language: English
Additional information: This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions.
Keywords: Ovarian cancer, Photochemical internalisation, Photodynamic therapy, 3D compressed collagen construct, Spheroid
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Surgery and Interventional Sci
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Surgery and Interventional Sci > Department of Surgical Biotechnology
URI: https://discovery.ucl.ac.uk/id/eprint/10057053
Downloads since deposit
139Downloads
Download activity - last month
Download activity - last 12 months
Downloads by country - last 12 months

Archive Staff Only

View Item View Item