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Three dimensional characterisation of chromatography bead internal structure using X-ray computed tomography and focused ion beam microscopy

Johnson, TF; Bailey, JJ; Iacoviello, F; Welsh, JH; Levison, PR; Shearing, PR; Bracewell, DG; (2018) Three dimensional characterisation of chromatography bead internal structure using X-ray computed tomography and focused ion beam microscopy. Journal of Chromatography A , 1566 pp. 79-88. 10.1016/j.chroma.2018.06.054. Green open access

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Abstract

X-ray computed tomography (CT) and focused ion beam (FIB) microscopy were used to generate three dimensional representations of chromatography beads for quantitative analysis of important physical characteristics including tortuosity factor. Critical-point dried agarose, cellulose and ceramic beads were examined using both methods before digital reconstruction and geometry based analysis for comparison between techniques and materials examined. X-ray ‘nano’ CT attained a pixel size of 63 nm and 32 nm for respective large field of view and high resolution modes. FIB improved upon this to a 15 nm pixel size for the more rigid ceramic beads but required compromises for the softer agarose and cellulose materials, especially during physical sectioning that was not required for X-ray CT. Digital processing of raw slices was performed using software to produce 3D representations of bead geometry. Porosity, tortuosity factor, surface area to volume ratio and pore diameter were evaluated for each technique and material, with overall averaged simulated tortuosity factors of 1.36, 1.37 and 1.51 for agarose, cellulose and ceramic volumes respectively. Results were compared to existing literature values acquired using established imaging and non-imaging techniques to demonstrate the capability of tomographic approaches used here.

Type: Article
Title: Three dimensional characterisation of chromatography bead internal structure using X-ray computed tomography and focused ion beam microscopy
Open access status: An open access version is available from UCL Discovery
DOI: 10.1016/j.chroma.2018.06.054
Publisher version: http://doi.org/10.1016/j.chroma.2018.06.054
Language: English
Additional information: Copyright © 2018 The Authors. Published by Elsevier B.V.This is an open access article under the CCBY license(http://creativecommons.org/licenses/by/4.0/)
Keywords: Bead scale, X-ray computed tomography, Focused ion beam microscopy, Structure, Tortuosity
UCL classification: UCL
UCL > Provost and Vice Provost Offices > UCL BEAMS
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Biochemical Engineering
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Chemical Engineering
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Electronic and Electrical Eng
URI: https://discovery.ucl.ac.uk/id/eprint/10056564
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