De Castro, SCP;
Gustavsson, P;
Marshall, AR;
Gordon, WM;
Galea, G;
Nikolopoulou, E;
Savery, D;
... Greene, NDE; + view all
(2018)
Over-expression of Grainyhead-like 3 causes spina bifida and interacts genetically with mutant alleles of Grhl2 and Vangl2 in mice.
Human Molecular Genetics
, 27
(24)
pp. 4218-4230.
10.1093/hmg/ddy313.
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Abstract
The genetic basis of human neural tube defects (NTDs), such as anencephaly and spina bifida, is complex and heterogeneous. Grainyhead-like genes represent candidates for involvement in NTDs based on the presence of spina bifida and exencephaly in mice carrying loss-of-function alleles of Grhl2 or Grhl3. We found that reinstatement of Grhl3 expression, by BAC-mediated transgenesis, prevents spina bifida in Grhl3 null embryos, as in the Grhl3 hypomorphic curly tail strain. Notably however, further increase in expression of Grhl3 causes highly penetrant spina bifida. Grhl3 over-expression recapitulates the spinal NTD phenotype of loss-of-function embryos, although the underlying mechanism differs. However, it does not phenocopy other defects of Grhl3 null embryos such as abnormal axial curvature, cranial NTDs (exencephaly) or skin barrier defects, the latter being rescued by the Grhl3-transgene. Grhl2 and Grhl3 can form homo- and heterodimers, suggesting a possible model in which defects arising from over-expression of Grhl3 result from sequestration of Grhl2 in heterodimers, mimicking Grhl2 loss of function. This hypothesis predicts that increased abundance of Grhl2 would have an ameliorating effect in Grhl3 over-expressing embryo. Instead we observed a striking additive genetic interaction between Grhl2 and Grhl3 gain-of-function alleles. Severe spina bifida arose in embryos in which both genes were expressed at moderately elevated levels that individually do not cause NTDs. Furthermore, moderate Grhl3 over-expression also interacted with the Vangl2Lp allele to cause spina bifida, demonstrating genetic interaction with the planar cell polarity signalling pathway that is implicated in mouse and human NTDs.
| Type: | Article |
|---|---|
| Title: | Over-expression of Grainyhead-like 3 causes spina bifida and interacts genetically with mutant alleles of Grhl2 and Vangl2 in mice. |
| Location: | England |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1093/hmg/ddy313 |
| Publisher version: | https://doi.org/10.1093/hmg/ddy313 |
| Language: | English |
| Additional information: | This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. |
| Keywords: | alleles, phenotype, embryo, genes, cranium, spinal dysraphism, transgenes, mice, skin, protein over expression, gene interaction |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > UCL GOS Institute of Child Health UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > UCL GOS Institute of Child Health > Developmental Biology and Cancer Dept |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10056431 |
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