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Biocytin Recovery and 3D Reconstructions of Filled Hippocampal CA2 Interneurons

Economides, G; Falk, S; Mercer, A; (2018) Biocytin Recovery and 3D Reconstructions of Filled Hippocampal CA2 Interneurons. Journal of Visualized Experiments , 141 , Article e58592. 10.3791/58592. Green open access

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Abstract

How cortical network activity processes information is of importance to a large number of basic 26 and clinical scientific questions. The protocol described here identifies the basic building blocks 27 of this circuitry. The in-depth studies of cortical regions will ultimately provide other scientists 28 with the circuit components needed for an understanding of how the brain acquires, processes 29 and stores information and what goes wrong in disease, while the electrophysiological and morphological data are widely used by computational neuroscientists in the construction of model networks that explore information processing. The protocol outlined here describes how biocytin-filled cells recorded in the CA2 region of the hippocampus are recovered and then reconstructed in 3D. Additionally, the protocol describes the demonstration of calcium binding protein or peptide content in recorded interneurons.

Type: Article
Title: Biocytin Recovery and 3D Reconstructions of Filled Hippocampal CA2 Interneurons
Open access status: An open access version is available from UCL Discovery
DOI: 10.3791/58592
Publisher version: http://dx.doi.org/10.3791/58592
Language: English
Additional information: This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Keywords: Immunohistochemistry, immunofluorescence protocol, HRP protocol, neuronal reconstructions, Neurolucida, Camera Lucida, neuronal anatomy, dendrites, axon
UCL classification: UCL
UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > UCL School of Pharmacy
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > UCL School of Pharmacy > Pharmacology
URI: https://discovery.ucl.ac.uk/id/eprint/10056410
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